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. 1981 Nov;148(2):498–507. doi: 10.1128/jb.148.2.498-507.1981

Cloning of immunity and structural genes for colicin V.

K K Frick, R L Quackenbush, J Konisky
PMCID: PMC216232  PMID: 6271732

Abstract

The colicin V immunity and structural genes of plasmid pColV-B188 were cloned into the vectors pMB9, pBR322, and pMK16. Both genes are closely linked and can be isolated on a 900-base-pair deoxyribonucleic acid fragment. Insertion of the transposon Tn5 into this cloned sequence led to the construction of a mutant plasmid which conferred colicin V immunity, but not the ability to produce this colicin. Analysis of the products determined by these cloned genes in cells has led to the conclusion that the polypeptide involved in immunity has a molecular weight of about 6,500, whereas the colicin has a molecular weight of approximately 4,000.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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