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. 1981 Nov;148(2):508–513. doi: 10.1128/jb.148.2.508-513.1981

Effects of growth substrate and respiratory chain composition on bioenergetics in Acinetobacter sp. strain HO1-N.

B D Ensley, R M Irwin, L A Carreira, P S Hoffman, T V Morgan, W R Finnerty
PMCID: PMC216233  PMID: 6271733

Abstract

The relationship between respiratory chain composition and efficiency of coupling phosphorylation to electron transport was examined in Acinetobacter sp. strain HO1-N. Cells containing only cytochrome o as a terminal oxidase displayed the same stoichiometries of adenosine 5'-triphosphate synthesis and proton extrusion as cells which contained both cytochromes o and d as terminal oxidases. In addition, CO inhibition and photo-relief of cytochromes o or d did not alter the efficiency of energy coupling. These findings indicate that adenosine 5'-triphosphate synthesis is coupled to electron transport through both cytochromes o and d in Acinetobacter.

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Selected References

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  1. Ackrell B. A., Jones C. W. The respiratory system of Azotobacter vinelandii. 1. Properties of phosphorylating respiratory membranes. Eur J Biochem. 1971 May 11;20(1):22–28. doi: 10.1111/j.1432-1033.1971.tb01357.x. [DOI] [PubMed] [Google Scholar]
  2. Arima K., Oka T. Cyanide Resistance in Achromobacter I. Induced Formation of Cytochrome a(2) and Its Role in Cyanide-Resistant Respiration. J Bacteriol. 1965 Sep;90(3):734–743. doi: 10.1128/jb.90.3.734-743.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ashcroft J. R., Haddock B. A. Synthesis of alternative membrane-bound redox carriers during aerobic growth of Escherichia coli in the presence of potassium cyanide. Biochem J. 1975 May;148(2):349–352. doi: 10.1042/bj1480349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baumann P., Doudoroff M., Stanier R. Y. A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J Bacteriol. 1968 May;95(5):1520–1541. doi: 10.1128/jb.95.5.1520-1541.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CASTOR L. N., CHANCE B. Photochemical determinations of the oxidases of bacteria. J Biol Chem. 1959 Jun;234(6):1587–1592. [PubMed] [Google Scholar]
  6. CHANCE B. The carbon monoxide compounds of the cytochrome oxidases. II. Photodissociation spectra. J Biol Chem. 1953 May;202(1):397–406. [PubMed] [Google Scholar]
  7. Downs A. J., Jones C. W. Respiration-linked proton translocation in Azotobacter vinelandii. FEBS Lett. 1975 Dec 1;60(1):42–46. doi: 10.1016/0014-5793(75)80414-5. [DOI] [PubMed] [Google Scholar]
  8. Ensley B. D., Jr, Finnerty W. R. Influences of growth substrates and oxygen on the electron transport system in Acinetobacter sp. HO1-N. J Bacteriol. 1980 Jun;142(3):859–868. doi: 10.1128/jb.142.3.859-868.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fernandes R., Jones M., King H. K. Purification and properties of malate-NAD+ dehydrogenase of Moraxella lwoffi (N.C.I.B. 8250). Biochem Soc Trans. 1976;4(6):1080–1080. doi: 10.1042/bst0041080. [DOI] [PubMed] [Google Scholar]
  10. Hoffman P. S., Irwin R. M., Carreira L. A., Morgan T. V., Ensley B. D., Dervartanian D. V. Studies of photochemical action spectra on N,N,N',N'-tetramethyl-p-phenylenediamine-oxidase-negative mutants of Azotobacter vinelandii. Eur J Biochem. 1980 Mar;105(1):177–185. doi: 10.1111/j.1432-1033.1980.tb04487.x. [DOI] [PubMed] [Google Scholar]
  11. Jones C. W., Brice J. M., Downs A. J., Drozd J. W. Bacterial respiration-linked proton translocation and its relationship to respiratory-chain composition. Eur J Biochem. 1975 Mar 17;52(2):265–271. doi: 10.1111/j.1432-1033.1975.tb03994.x. [DOI] [PubMed] [Google Scholar]
  12. Juni E. Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. J Bacteriol. 1972 Nov;112(2):917–931. doi: 10.1128/jb.112.2.917-931.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KASHKET E. R., BRODIE A. F. OXIDATIVE PHOSPHORYLATION IN FRACTIONATED BACTERIAL SYSTEMS. VIII. ROLE OF PARTICULATE AND SOLUBLE FRACTIONS FROM ESCHERICHIA COLI. Biochim Biophys Acta. 1963 Oct 8;78:52–65. doi: 10.1016/0006-3002(63)91608-1. [DOI] [PubMed] [Google Scholar]
  14. Knowles C. J., Smith L. Measurements of ATP levels of intact Azotobacter vinelandii under different conditions. Biochim Biophys Acta. 1970 Mar 3;197(2):152–160. doi: 10.1016/0005-2728(70)90026-5. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lawford H. G., Haddock B. A. Respiration-driven proton translocation in Escherichia coli. Biochem J. 1973 Sep;136(1):217–220. doi: 10.1042/bj1360217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Meyer D. J., Jones C. W. Oxidative phosphorylation in bacteria which contain different cytochrome oxidases. Eur J Biochem. 1973 Jul 2;36(1):144–151. doi: 10.1111/j.1432-1033.1973.tb02894.x. [DOI] [PubMed] [Google Scholar]
  18. Mitchell P. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966 Aug;41(3):445–502. doi: 10.1111/j.1469-185x.1966.tb01501.x. [DOI] [PubMed] [Google Scholar]
  19. Mitchell P., Moyle J. Acid-base titration across the membrane system of rat-liver mitochondria. Catalysis by uncouplers. Biochem J. 1967 Aug;104(2):588–600. doi: 10.1042/bj1040588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mitchell P., Moyle J. Respiration-driven proton translocation in rat liver mitochondria. Biochem J. 1967 Dec;105(3):1147–1162. doi: 10.1042/bj1051147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. PINCHOT G. B. A polynucleotide coenzyme of oxidative phosphorylation. II. J Biol Chem. 1957 Nov;229(1):25–37. [PubMed] [Google Scholar]
  22. Payne W. J., Wiebe W. J. Growth yield and efficiency in chemosynthetic microorganisms. Annu Rev Microbiol. 1978;32:155–183. doi: 10.1146/annurev.mi.32.100178.001103. [DOI] [PubMed] [Google Scholar]
  23. Rice C. W., Hempfling W. P. Oxygen-limited continuous culture and respiratory energy conservation in Escherichia coli. J Bacteriol. 1978 Apr;134(1):115–124. doi: 10.1128/jb.134.1.115-124.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Scholes P., Mitchell P. Respiration-driven proton translocation in Micrococcus denitrificans. J Bioenerg. 1971 Sep;1(3):309–323. doi: 10.1007/BF01516290. [DOI] [PubMed] [Google Scholar]
  25. Scott C. C., Makula S. R., Finnerty W. R. Isolation and characterization of membranes from a hydrocarbon-oxidizing Acinetobacter sp. J Bacteriol. 1976 Jul;127(1):469–480. doi: 10.1128/jb.127.1.469-480.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sinclair P. R., White D. C. Effect of nitrate, fumarate, and oxygen on the formation of the membrane-bound electron transport system of Haemophilus parainfluenzae. J Bacteriol. 1970 Feb;101(2):365–372. doi: 10.1128/jb.101.2.365-372.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WHITE D. C. FACTORS AFFECTING THE AFFINITY FOR OXYGEN OF CYTOCHROME OXIDASES IN HEMOPHILUS PARAINFLUENZAE. J Biol Chem. 1963 Nov;238:3757–3761. [PubMed] [Google Scholar]
  28. White D. C. Effect of glucose on the formation of the membrane-bound electron transport system in Haemophilus parainfluenzae. J Bacteriol. 1967 Feb;93(2):567–573. doi: 10.1128/jb.93.2.567-573.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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