Abstract
Treatment of either mating type of Saccharomyces cerevisiae with the appropriate sex pheromone increased cell-cell binding in a modified cocentrifugation assay. Constitutive agglutination of haploids was qualitatively similar to pheromone-induced agglutination. Regardless of exposure to pheromone, agglutinable combinations of cells exhibited maximal binding across similar ranges of ionic strength, pH, and temperature. Binding of all combinations was inhibited by 8 M urea, 1 M pyridine, or 0.05% sodium dodecyl sulfate. From alpha-cells we solubilized and partially purified an inhibitor of a-cell agglutinability. This inhibitor reversibly masked all a-cell adhesion sites and inactivated pheromone-treated and control cells with similar kinetics. The inhibitor behaved as a homogeneous species in heat inactivation experiments. Based on these results, we proposed a model for pheromone effects on agglutination in S. cerevisiae.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Campbell D. A. Kinetics of the mating-specific aggregation in Saccharomyces cerevisiae. J Bacteriol. 1973 Oct;116(1):323–330. doi: 10.1128/jb.116.1.323-330.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duntze W., Stötzler D., Bücking-Throm E., Kalbitzer S. Purification and partial characterization of -factor, a mating-type specific inhibitor of cell reproduction from Saccharomyces cerevisiae. Eur J Biochem. 1973 Jun;35(2):357–365. doi: 10.1111/j.1432-1033.1973.tb02847.x. [DOI] [PubMed] [Google Scholar]
- Fehrenbacher G., Perry K., Thorner J. Cell-cell recognition in Saccharomyces cerevisiae: regulation of mating-specific adhesion. J Bacteriol. 1978 Jun;134(3):893–901. doi: 10.1128/jb.134.3.893-901.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartwell L. H. Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone. J Cell Biol. 1980 Jun;85(3):811–822. doi: 10.1083/jcb.85.3.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipke P. N., Ballou C. E. Altered immunochemical reactivity of Saccharomyces cerevisiae a-cells after alpha-factor-induced morphogenesis. J Bacteriol. 1980 Mar;141(3):1170–1177. doi: 10.1128/jb.141.3.1170-1177.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipke P. N., Taylor A., Ballou C. E. Morphogenic effects of alpha-factor on Saccharomyces cerevisiae a cells. J Bacteriol. 1976 Jul;127(1):610–618. doi: 10.1128/jb.127.1.610-618.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimoda C., Kitano S., Yanagishima N. Mating reaction in Saccharomyces cerevisiae. VII. Effect of proteolytic enzymes on sexual agglutinability and isolation of crude sex-specific substances responsible for sexual agglutination. Antonie Van Leeuwenhoek. 1975;41(4):513–519. doi: 10.1007/BF02565093. [DOI] [PubMed] [Google Scholar]
- Smith W. L., Ballou C. E. Immunochemical characterization of the mannan component of the external invertase (beta-fructofuranosidase) of Saccharomyces cerevisiae. Biochemistry. 1974 Jan 15;13(2):355–361. doi: 10.1021/bi00699a021. [DOI] [PubMed] [Google Scholar]