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. 1981 Dec;148(3):919–925. doi: 10.1128/jb.148.3.919-925.1981

Adenosine 3',5'-phosphate phosphodiesterase and pheromone response in the yeast Saccharomyces cerevisiae.

H H Liao, J Thorner
PMCID: PMC216293  PMID: 6171560

Abstract

Theophylline, aminophylline, and isobutylmethylxanthine, compounds reported to be inhibitors of adenosine 3',5'-phosphate (cAMP) phosphodiesterase, prevented the alpha-factor-induced cell cycle arrest of Saccharomyces cerevisiae a cells. To determine whether the in vivo effect of these methylxanthines on yeast pheromone response was related to their known biochemical mode of action, two assays for cAMP phosphodiesterase based on affinity of the product of the reaction (5'-AMP) for boronate groups were developed and were used to monitor the activity of the low Km cAMP phosphodiesterase present in yeast extracts. It was found that the relative efficacy of the methylxanthines as inhibitors of this enzyme in vitro was correlated with the degree to which they antagonized alpha-factor action in vivo. These results were consistent with our previous proposal that pheromone action involves a lowering of cAMP level in the target cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bard M., Neuhauser J. L., Lees N. D. Caffeine resistance of Saccharomyces cerevisiae. J Bacteriol. 1980 Feb;141(2):999–1002. doi: 10.1128/jb.141.2.999-1002.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bücking-Throm E., Duntze W., Hartwell L. H., Manney T. R. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor. Exp Cell Res. 1973 Jan;76(1):99–110. doi: 10.1016/0014-4827(73)90424-2. [DOI] [PubMed] [Google Scholar]
  3. Chan R. K. Recovery of Saccharomyces cerevisiae mating-type a cells from G1 arrest by alpha factor. J Bacteriol. 1977 May;130(2):766–774. doi: 10.1128/jb.130.2.766-774.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ciejek E., Thorner J., Geier M. Solid phase peptide synthesis of alpha-factor, a yeast mating pheromone. Biochem Biophys Res Commun. 1977 Oct 10;78(3):952–961. doi: 10.1016/0006-291x(77)90514-9. [DOI] [PubMed] [Google Scholar]
  5. Ciejek E., Thorner J. Recovery of S. cerevisiae a cells from G1 arrest by alpha factor pheromone requires endopeptidase action. Cell. 1979 Nov;18(3):623–635. doi: 10.1016/0092-8674(79)90117-x. [DOI] [PubMed] [Google Scholar]
  6. Davis C. W., Daly J. W. A simple direct assay of 3',5'-cyclic nucleotide phosphodiesterase activity based on the use of polyacrylamide-bononate affinity gel chromatography. J Cyclic Nucleotide Res. 1979;5(1):65–74. [PubMed] [Google Scholar]
  7. Fehrenbacher G., Perry K., Thorner J. Cell-cell recognition in Saccharomyces cerevisiae: regulation of mating-specific adhesion. J Bacteriol. 1978 Jun;134(3):893–901. doi: 10.1128/jb.134.3.893-901.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hageman J. H., Kuehn G. D. Assay of adenylate cyclase by use of polyacrylamide--boronate gel columns. Anal Biochem. 1977 Jun;80(2):547–554. doi: 10.1016/0003-2697(77)90678-9. [DOI] [PubMed] [Google Scholar]
  9. Liao H., Thorner J. Yeast mating pheromone alpha factor inhibits adenylate cyclase. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1898–1902. doi: 10.1073/pnas.77.4.1898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Londesborough J. C. Soluble and membrane-bound cyclic AMP diesterase activity with a low Michaelis constant in baker's yeast. FEBS Lett. 1975 Feb 1;50(2):283–287. doi: 10.1016/0014-5793(75)80509-6. [DOI] [PubMed] [Google Scholar]
  11. Londesborough J. Characterization of an adenosine 3':5'-cyclic monophosphate phosphodiesterase from baker's yeast. Its binding to subcellular particles, catalytic properties and gel-filtration behaviour. Biochem J. 1977 Jun 1;163(3):467–476. doi: 10.1042/bj1630467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Londesborough J., Lukkari T. M. The pH and temperature dependence of the activity of the high Km cyclic nucleotide phosphodiesterase of bakers' yeast. J Biol Chem. 1980 Oct 10;255(19):9262–9267. [PubMed] [Google Scholar]
  13. Londesborough J. Quantitative estimation of 3'5' cyclic AMP phosphodiesterase using anion exchange resin in a batch process. Anal Biochem. 1976 Apr;71(2):623–628. doi: 10.1016/s0003-2697(76)80037-1. [DOI] [PubMed] [Google Scholar]
  14. Londesborough J. The high-Km cyclic AMP phosphodiesterase of baker's yeast is a zinc metalloenzyme [proceedings]. Biochem Soc Trans. 1978;6(6):1218–1220. doi: 10.1042/bst0061218. [DOI] [PubMed] [Google Scholar]
  15. Moore E. C., Peterson D., Yang L. Y., Yeung C. Y., Neff N. F. Separation of ribonucleotides and deoxyribonucleotides on columns of borate covalently linked to cellulose. Application to the assay of ribonucleoside diphosphate reductase. Biochemistry. 1974 Jul 2;13(14):2904–2907. doi: 10.1021/bi00711a020. [DOI] [PubMed] [Google Scholar]
  16. Pastan I. H., Johnson G. S., Anderson W. B. Role of cyclic nucleotides in growth control. Annu Rev Biochem. 1975;44:491–522. doi: 10.1146/annurev.bi.44.070175.002423. [DOI] [PubMed] [Google Scholar]
  17. Schlanderer G., Dellweg H. Cyclid AMP and catabolite repression in yeasts, In Schizosaccharomyces pombe glucose lowers both intracellular adenosine 3':5'-monophosphate levels and the activity of catabolite-sensitive enzymes. Eur J Biochem. 1974 Nov 1;49(1):305–316. doi: 10.1111/j.1432-1033.1974.tb03835.x. [DOI] [PubMed] [Google Scholar]
  18. Sy J., Richter D. Content of cyclic 3',5'-adenosine monophosphate and adenylyl cyclase in yeast at various growth conditions. Biochemistry. 1972 Jul 18;11(15):2788–2791. doi: 10.1021/bi00765a009. [DOI] [PubMed] [Google Scholar]
  19. Van Wijk R., Konijn T. M. Cyclic 3', 5'-amp in Saccharomyces carlsbergensis under various conditions of catabolite repression. FEBS Lett. 1971 Mar 5;13(3):184–186. doi: 10.1016/0014-5793(71)80231-4. [DOI] [PubMed] [Google Scholar]
  20. Weith H. L., Wiebers J. L., Gilham P. T. Synthesis of cellulose derivatives containing the dihydroxyboryl group and a study of their capacity to form specific complexes with sugars and nucleic acid components. Biochemistry. 1970 Oct 27;9(22):4396–4401. doi: 10.1021/bi00824a021. [DOI] [PubMed] [Google Scholar]
  21. Zusman D. R. A rapid batch assay for cyclic AMP phosphodiesterase. Anal Biochem. 1978 Feb;84(2):551–558. doi: 10.1016/0003-2697(78)90074-x. [DOI] [PubMed] [Google Scholar]

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