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. 1982 Jun;150(3):1322–1328. doi: 10.1128/jb.150.3.1322-1328.1982

Efficiency of light-driven metabolite transport in the photosynthetic bacterium Rhodospirillum rubrum.

M Zebrower, P A Loach
PMCID: PMC216357  PMID: 6804443

Abstract

An evaluation of the efficiency of the L-alanine and L-malate transport systems was undertaken with the photosynthetic bacterium Rhodospirillum rubrum grown on the amino acid whose uptake was measured. An all-glass apparatus was constructed for measuring transport activity under anaerobic conditions. L-Alanine transport activity decreased under conditions of Mg2+ depletion. When cells were allowed to become inactive by suspending them in the dark in Mg2+-free buffer, full activity could be restored with a few minutes by adding 20 mM Mg2+ and illuminating the cells. The transport activity was completely inhibited by carbonyl cyanide m-trifluoromethoxyphenylhydrazone and by ammonia. The quantum yield for the uptake of either L-alanine or L-malate was 0.015 molecules per photon. The results are discussed in relation to the expected efficiencies for metabolite transport and regulation by Mg2+.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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