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. 1982 May;150(2):490–497. doi: 10.1128/jb.150.2.490-497.1982

Siderophore production by Enterobacter cloacae and a common receptor protein for the uptake of aerobactin and cloacin DF13.

G J Van Tiel-Menkveld, J M Mentjox-Vervuurt, B Oudega, F K de Graaf
PMCID: PMC216393  PMID: 6461633

Abstract

Iron-starved cultures of Enterobacter cloacae produced two siderophores, identified as enterochelin and aerobactin. The aerobactin was excreted in larger amounts than was enterochelin, and it was synthesized preferentially in the late logarithmic and stationary growth phases under iron-deficient conditions. Enterochelin was synthesized by cultures in the logarithmic phase of growth and preferentially in medium with 1 microM ferric chloride. Both siderophores appeared to be excreted immediately after their synthesis, since no intracellular aerobactin or enterochelin could be detected. The killing activity of the bacteriocin cloacin DF13 was inhibited by aerobactin. It was shown that aerobactin and cloacin DF13 bound to the same receptor sites located in the outer membrane. The synthesis of these receptor sites was induced by iron limitation. We conclude that the receptor for the uptake of aerobactin also functions as receptor for cloacin DF13.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arceneaux J. E., Davis W. B., Downer D. N., Haydon A. H., Byers B. R. Fate of labeled hydroxamates during iron transport from hydroxamate-ion chelates. J Bacteriol. 1973 Sep;115(3):919–927. doi: 10.1128/jb.115.3.919-927.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassford P. J., Jr, Kadner R. J., Schnaitman C. A. Biosynthesis of the outer membrane receptor for vitamin B12, E colicins, and bacteriophage BF23 by Escherichia coli: kinetics of phenotypic expression after the introduction of bfe+ and bfe alleles. J Bacteriol. 1977 Jan;129(1):265–275. doi: 10.1128/jb.129.1.265-275.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. De Graaf F. K., Klaasen-Boor P. Purification and characterization of a complex between cloacin and its immunity protein isolated from Enterobacter cloacae (Clo DF13). Dissociation and reconstitution of the complex. Eur J Biochem. 1977 Feb 15;73(1):107–114. doi: 10.1111/j.1432-1033.1977.tb11296.x. [DOI] [PubMed] [Google Scholar]
  4. Gibson F., Magrath D. I. The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I. Biochim Biophys Acta. 1969 Nov 18;192(2):175–184. doi: 10.1016/0304-4165(69)90353-5. [DOI] [PubMed] [Google Scholar]
  5. Guterman S. K. Colicin B: mode of action and inhibition by enterochelin. J Bacteriol. 1973 Jun;114(3):1217–1224. doi: 10.1128/jb.114.3.1217-1224.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hancock R. E., Hantke K., Braun V. Iron transport of Escherichia coli K-12: involvement of the colicin B receptor and of a citrate-inducible protein. J Bacteriol. 1976 Sep;127(3):1370–1375. doi: 10.1128/jb.127.3.1370-1375.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hantke K., Braun V. Membrane receptor dependent iron transport in Escherichia coli. FEBS Lett. 1975 Jan 1;49(3):301–305. doi: 10.1016/0014-5793(75)80771-x. [DOI] [PubMed] [Google Scholar]
  8. Hartmann A., Braun V. Iron transport in Escherichia coli: uptake and modification of ferrichrome. J Bacteriol. 1980 Jul;143(1):246–255. doi: 10.1128/jb.143.1.246-255.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollifield W. C., Jr, Neilands J. B. Ferric enterobactin transport system in Escherichia coli K-12. Extraction, assay, and specificity of the outer membrane receptor. Biochemistry. 1978 May 16;17(10):1922–1928. doi: 10.1021/bi00603a019. [DOI] [PubMed] [Google Scholar]
  10. Kadner R. J., McElhaney G. Outer membrane-dependent transport systems in Escherichia coli: effect of repression or cessation of colicin receptor synthesis on colicin receptor activities. J Bacteriol. 1980 Jul;143(1):135–141. doi: 10.1128/jb.143.1.135-141.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Brien I. G., Cox G. B., Gibson F. Biologically active compounds containing 2,3-dihydroxybenzoic acid and serine formed by Escherichia coli. Biochim Biophys Acta. 1970 Mar 24;201(3):453–460. doi: 10.1016/0304-4165(70)90165-0. [DOI] [PubMed] [Google Scholar]
  12. O'Brien I. G., Gibson F. The structure of enterochelin and related 2,3-dihydroxy-N-benzoylserine conjugates from Escherichia coli. Biochim Biophys Acta. 1970 Aug 14;215(2):393–402. doi: 10.1016/0304-4165(70)90038-3. [DOI] [PubMed] [Google Scholar]
  13. Oudega B., Klaasen-Boor P., Sneeuwloper G., De Graaf F. K. Interaction of the complex between cloacin and its immunity protein and of cloacin with the outer and cytoplasmic membranes of sensitive cells. Eur J Biochem. 1977 Sep;78(2):445–453. doi: 10.1111/j.1432-1033.1977.tb11757.x. [DOI] [PubMed] [Google Scholar]
  14. Oudega B., Oldenziel-Werner W. J., Klaasen-Boor P., Rezee A., Glas J., de Graaf F. K. Purification and characterization of cloacin DF13 receptor from Enterobacter cloacae and its interaction with cloacin DF13 in vitro. J Bacteriol. 1979 Apr;138(1):7–16. doi: 10.1128/jb.138.1.7-16.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Oudega B., van der Molen J., de Graaf F. K. In vitro binding of cloacin DF13 to its purified outer membrane receptor protein and effect of peptidoglycan on bacteriocin-receptor interaction. J Bacteriol. 1979 Dec;140(3):964–970. doi: 10.1128/jb.140.3.964-970.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Payne S. M. Synthesis and utilization of siderophores by Shigella flexneri. J Bacteriol. 1980 Sep;143(3):1420–1424. doi: 10.1128/jb.143.3.1420-1424.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perry R. D., San Clemente C. L. Siderophore synthesis in Klebsiella pneumoniae and Shigella sonnei during iron deficiency. J Bacteriol. 1979 Dec;140(3):1129–1132. doi: 10.1128/jb.140.3.1129-1132.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pollack J. R., Neilands J. B. Enterobactin, an iron transport compound from Salmonella typhimurium. Biochem Biophys Res Commun. 1970 Mar 12;38(5):989–992. doi: 10.1016/0006-291x(70)90819-3. [DOI] [PubMed] [Google Scholar]
  19. Pugsley A. P., Reeves P. Iron uptake in colicin B-resistant mutants of Escherichia coli K-12. J Bacteriol. 1976 Jun;126(3):1052–1062. doi: 10.1128/jb.126.3.1052-1062.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shannon R., Hedges A. J. Reversibility of the specific adsorption of colicin E2-P9 to cells of colicin-sensitive strains of Escherichia coli. J Bacteriol. 1973 Dec;116(3):1136–1144. doi: 10.1128/jb.116.3.1136-1144.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stuart S. J., Greenwood K. T., Luke R. K. Hydroxamate-mediated transport of iron controlled by ColV plasmids. J Bacteriol. 1980 Jul;143(1):35–42. doi: 10.1128/jb.143.1.35-42.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Van Tiel-Menkvled G. J., Rezee A., De Graaf F. K. Production and excretion of cloacin DF13 by Escherichia coli harboring plasmid CloDF13. J Bacteriol. 1979 Nov;140(2):415–423. doi: 10.1128/jb.140.2.415-423.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Warner P. J., Williams P. H., Bindereif A., Neilands J. B. ColV plasmid-specific aerobactin synthesis by invasive strains of Escherichia coli. Infect Immun. 1981 Aug;33(2):540–545. doi: 10.1128/iai.33.2.540-545.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wayne R., Frick K., Neilands J. B. Siderophore protection against colicins M, B, V, and Ia in Escherichia coli. J Bacteriol. 1976 Apr;126(1):7–12. doi: 10.1128/jb.126.1.7-12.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wayne R., Neilands J. B. Evidence for common binding sites for ferrichrome compounds and bacteriophage phi 80 in the cell envelope of Escherichia coli. J Bacteriol. 1975 Feb;121(2):497–503. doi: 10.1128/jb.121.2.497-503.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Williams P. H., Warner P. J. ColV plasmid-mediated, colicin V-independent iron uptake system of invasive strains of Escherichia coli. Infect Immun. 1980 Aug;29(2):411–416. doi: 10.1128/iai.29.2.411-416.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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