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. 1982 May;150(2):973–976. doi: 10.1128/jb.150.2.973-976.1982

Cadmium and manganese transport in Staphylococcus aureus membrane vesicles.

R D Perry, S Silver
PMCID: PMC216454  PMID: 7068540

Abstract

The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ and Cd2+ were competitive inhibitors of each other's transport, with Km's of 0.95 microM Mn2+ and 0.2 microM Cd2+. The kinetic parameters were nearly identical with vesicles prepared from sensitive and resistant cells, indicating that the cadA-encoded Cd2+ efflux system was inoperative in membrane vesicle preparations. Experiments with energy-inhibited cells indicated that the cadB gene product may bind Cd2+.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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