Abstract
RNA polymerase was purified from five species of Bacillus, including Bacillus subtilis. Each polymerase had a subunit composition analogous to that reported for B. subtilis, i.e., beta beta '2 alpha sigma delta omega 1 omega 2. The delta subunits from the B. subtilis and Bacillus thuringiensis polymerases were interchangeable, as judged from their effects on promoter selection in the polymerase binding assay.
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- Achberger E. C., Whiteley H. R. The interaction of Escherichia coli core RNA polymerase with specificity-determining subunits derived from unmodified and SP82-modified Bacillus subtilis RNA polymerase. J Biol Chem. 1980 Dec 25;255(24):11957–11964. [PubMed] [Google Scholar]
- Achberger E. C., Whiteley H. R. The role of the delta peptide of the Bacillus subtilis RNA polymerase in promoter selection. J Biol Chem. 1981 Jul 25;256(14):7424–7432. [PubMed] [Google Scholar]
- Burgess R. R., Jendrisak J. J. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry. 1975 Oct 21;14(21):4634–4638. doi: 10.1021/bi00692a011. [DOI] [PubMed] [Google Scholar]
- Chelm B. K., Romeo J. M., Brennan S. M., Geiduschek E. P. A transcriptional map of the bacteriophage SPO1 genome. III. A region of early and middle promoters (the gene 28 region). Virology. 1981 Jul 30;112(2):572–588. doi: 10.1016/0042-6822(81)90303-2. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Mangel W. F., Chamberlin M. J. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. I. An assay for the rate and extent of ribonucleic acid chain initiation. J Biol Chem. 1974 May 25;249(10):2995–3001. [PubMed] [Google Scholar]
- Spiegelman G. B., Hiatt W. R., Whiteley H. R. Role of the 21,000 molecular weight polypeptide of Bacillus subtilis RNA polymerase in RNA synthesis. J Biol Chem. 1978 Mar 25;253(6):1756–1765. [PubMed] [Google Scholar]
- Spiegelman G. B., Whiteley H. R. Bacteriophage SP82 induced modifications of Bacillus subtilis RNA polymerase result in the recognition of additional RNA synthesis initiation sites on phage DNA. Biochem Biophys Res Commun. 1978 Apr 14;81(3):1058–1065. doi: 10.1016/0006-291x(78)91458-4. [DOI] [PubMed] [Google Scholar]
- Talkington C., Pero J. Distinctive nucleotide sequences of promoters recognized by RNA polymerase containing a phage-coded "sigma-like" protein. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5465–5469. doi: 10.1073/pnas.76.11.5465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tjian R., Losick R., Pero J., Hinnebush A. Purification and comparative properties of the delta and sigma subunits of RNA polymerase from Bacillus subtilis. Eur J Biochem. 1977 Mar 15;74(1):149–154. doi: 10.1111/j.1432-1033.1977.tb11376.x. [DOI] [PubMed] [Google Scholar]
- Whiteley H. R., Hemphill H. E. The interchangeability of stimulatory factors isolated from three microbial RNA polymerases. Biochem Biophys Res Commun. 1970 Nov 9;41(3):647–654. doi: 10.1016/0006-291x(70)90062-8. [DOI] [PubMed] [Google Scholar]
- Wiggs J. L., Bush J. W., Chamberlin M. J. Utilization of promoter and terminator sites on bacteriophage T7 DNA by RNA polymerases from a variety of bacterial orders. Cell. 1979 Jan;16(1):97–109. doi: 10.1016/0092-8674(79)90191-0. [DOI] [PubMed] [Google Scholar]