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. 1982 May;150(2):981–983. doi: 10.1128/jb.150.2.981-983.1982

Induction of acyl coenzyme A synthetase and hydroxyacyl coenzyme A dehydrogenase during fatty acid degradation in Neurospora crassa.

V Hii, J B Courtright
PMCID: PMC216456  PMID: 6461637

Abstract

Neurospora crassa is able to use long-chain fatty acids as the sole carbon and energy source. After growth on oleate there was nearly a 10-fold induction of the acyl coenzyme A (CoA) synthetase and a fivefold increase in the activity of the 3-hydroxyacyl-CoA dehydrogenase. There was a slight induction of the enoyl-CoA hydratase and 3-ketoacyl-CoA thiolase, but no apparent induction of the flavin-linked acyl-CoA dehydrogenase. These noncoordinate changes in the fatty acid degradation enzymes suggest that they are not organized into a multienzyme complex as is found in bacteria.

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Selected References

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  1. ARRIGONI O., SINGER T. P. Limitations of the phenazine methosulphate assay for succinic and related dehydrogenases. Nature. 1962 Mar 31;193:1256–1258. doi: 10.1038/1931256a0. [DOI] [PubMed] [Google Scholar]
  2. Brody S., Martins S. A. Circadian rhythms in Neurospora crassa: effects of unsaturated fatty acids. J Bacteriol. 1979 Feb;137(2):912–915. doi: 10.1128/jb.137.2.912-915.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clinkenbeard K. D., Sugiyama T., Moss J., Reed W. D., Lane M. D. Molecular and catalytic properties of cytosolic acetoacetyl coenzyme A thiolase from avian liver. J Biol Chem. 1973 Apr 10;248(7):2275–2284. [PubMed] [Google Scholar]
  4. Courtright J. B. Characteristics of a glycerol utilization mutant of Neurospora crassa. J Bacteriol. 1975 Oct;124(1):497–502. doi: 10.1128/jb.124.1.497-502.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Courtright J. B. Intracellular localization and properties of glycerokinase and glycerophosphate dehydrogenase in Neurospora crassa. Arch Biochem Biophys. 1975 Mar;167(1):21–33. doi: 10.1016/0003-9861(75)90437-3. [DOI] [PubMed] [Google Scholar]
  6. Elovson J. Purification and properties of the fatty acids synthetase complex from Neurospora crassa, and the nature of the fas-mutation. J Bacteriol. 1975 Oct;124(1):524–533. doi: 10.1128/jb.124.1.524-533.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green D. E., Allmann D. W., Harris R. A., Tan W. C. Enzyme localization in the inner and outer mitochondrial membranes. Biochem Biophys Res Commun. 1968 May 10;31(3):368–378. doi: 10.1016/0006-291x(68)90485-3. [DOI] [PubMed] [Google Scholar]
  8. Henry S. A., Keith A. D. Saturated fatty acid requirer of Neurospora crassa. J Bacteriol. 1971 Apr;106(1):174–182. doi: 10.1128/jb.106.1.174-182.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kung H. F., Henning U. Limiting availability of binding sites for dehydrogenases on the cell membrane of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Apr;69(4):925–929. doi: 10.1073/pnas.69.4.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LEIN J., LEIN P. S. The production of acetate from fatty acids by Neurospora. J Bacteriol. 1950 Aug;60(2):185–190. doi: 10.1128/jb.60.2.185-190.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Brien W. J., Frerman F. E. Evidence for a complex of three beta-oxidation enzymes in Escherichia coli: induction and localization. J Bacteriol. 1977 Nov;132(2):532–540. doi: 10.1128/jb.132.2.532-540.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Overath P., Raufuss E. M. The induction of the enzymes of fatty acid degradation in Escherichia coli. Biochem Biophys Res Commun. 1967 Oct 11;29(1):28–33. doi: 10.1016/0006-291x(67)90535-9. [DOI] [PubMed] [Google Scholar]
  13. Pauli G., Overath P. ato Operon: a highly inducible system for acetoacetate and butyrate degradation in Escherichia coli. Eur J Biochem. 1972 Sep 25;29(3):553–562. doi: 10.1111/j.1432-1033.1972.tb02021.x. [DOI] [PubMed] [Google Scholar]
  14. Pramanik A., Pawar S., Antonian E., Schulz H. Five different enzymatic activities are associated with the multienzyme complex of fatty acid oxidation from Escherichia coli. J Bacteriol. 1979 Jan;137(1):469–473. doi: 10.1128/jb.137.1.469-473.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Simons R. W., Egan P. A., Chute H. T., Nunn W. D. Regulation of fatty acid degradation in Escherichia coli: isolation and characterization of strains bearing insertion and temperature-sensitive mutations in gene fadR. J Bacteriol. 1980 May;142(2):621–632. doi: 10.1128/jb.142.2.621-632.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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