Abstract
In this paper we show that partial deprivation of a carbon source, a nitrogen source, or phosphate in the presence of all other nutrients needed for growth initiates meiosis and sporulation of Saccharomyces cerevisiae homothallic strain Y55. For carbon deprivation experiments, cells were grown in synthetic medium (pH 5.5) containing an excess of one carbon source and then transferred to the same medium containing different concentrations of the same carbon source. In the case of transfer to different acetate concentrations, the log optical density at 600 nm increased at the previous rate until the cells had used up all of the acetate, whereupon the cells entered a stationary phase and did not sporulate. The same was observed with ethanol. In contrast, at different concentrations of dihydroxy-acetone or pyruvate, cells grew at different rates and sporulated optimally at intermediate concentrations (50 to 75 mM). The response to galactose was similar but reflected the presence of a low-affinity galactose transport system and the induction of a high-affinity galactose transport system. Cells could also sporulate when a glucose medium ran out of glucose, apparently because they initiated sporulation during the subsequent lag period and then used the produced ethanol as a carbon source. For phosphate deprivation experiments, cells growing with excess ethanol or pyruvate and phosphate were transferred to the same medium containing limiting amounts of phosphate. First, they used up the intracellular phosphate reserves for rapid growth, and then they sporulated optimally when an intermediate concentration (30 μM) of phosphate had been added to the medium. For nitrogen deprivation experiments, cells grown with excess acetate, ethanol, or pyruvate and NH4+ were transferred to the same medium from which all nitrogen had been removed. These cells sporulated well in acetate medium but poorly in ethanol and pyruvate media. However, the sporulation frequency in the latter media could be increased greatly by adding intermediate concentrations (1 mM) of the slowly metabolizable amino acids glycine, histidine, or phenylalanine. If one assumes that the sporulation response to partial deprivation of carbon-, nitrogen-, or phosphorus-containing compounds reflects control by a single metabolite, the intracellular concentration of this metabolite may decide at the START position (G1 phase) of the cell cycle whether a/α cells enter mitosis or meiosis.
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- Cirillo V. P. Galactose transport in Saccharomyces cerevisiae. I. Nonmetabolized sugars as substrates and inducers of the galactose transport system. J Bacteriol. 1968 May;95(5):1727–1731. doi: 10.1128/jb.95.5.1727-1731.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOUGLAS H. C., CONDIE F. The genetic control of galactose utilization in Saccharomyces. J Bacteriol. 1954 Dec;68(6):662–670. doi: 10.1128/jb.68.6.662-670.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOUGLAS H. C., HAWTHORNE D. C. ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES. Genetics. 1964 May;49:837–844. doi: 10.1093/genetics/49.5.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haber J. E., Halvorson H. O. Cell cycle dependency of sporulation in Saccharomyces cerevisiae. J Bacteriol. 1972 Mar;109(3):1027–1033. doi: 10.1128/jb.109.3.1027-1033.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartwell L. H. Saccharomyces cerevisiae cell cycle. Bacteriol Rev. 1974 Jun;38(2):164–198. doi: 10.1128/br.38.2.164-198.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirschberg J., Simchen G. Commitment to the mitotic cell cycle in yeast in relation to meiosis. Exp Cell Res. 1977 Mar 15;105(2):245–252. doi: 10.1016/0014-4827(77)90122-7. [DOI] [PubMed] [Google Scholar]
- Hopper A. K., Magee P. T., Welch S. K., Friedman M., Hall B. D. Macromolecule synthesis and breakdown in relation to sporulation and meiosis in yeast. J Bacteriol. 1974 Aug;119(2):619–628. doi: 10.1128/jb.119.2.619-628.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kane S. M., Roth R. Carbohydrate metabolism during ascospore development in yeast. J Bacteriol. 1974 Apr;118(1):8–14. doi: 10.1128/jb.118.1.8-14.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kotyk A. Properties of the sugar carrier in baker's yeast. II. Specificity of transport. Folia Microbiol (Praha) 1967;12(2):121–131. doi: 10.1007/BF02896872. [DOI] [PubMed] [Google Scholar]
- Kraig E., Haber J. E. Messenger ribonucleic acid and protein metabolism during sporulation of Saccharomyces cerevisiae. J Bacteriol. 1980 Dec;144(3):1098–1112. doi: 10.1128/jb.144.3.1098-1112.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lopez J. M., Uratani-Wong B., Freese E. Catabolite repression of enzyme synthesis does not prevent sporulation. J Bacteriol. 1980 Mar;141(3):1447–1449. doi: 10.1128/jb.141.3.1447-1449.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MILLER J. J. The metabolism of yeast sporulation. II. Stimulation and inhibition by monosaccharides. Can J Microbiol. 1957 Feb;3(1):81–90. doi: 10.1139/m57-010. [DOI] [PubMed] [Google Scholar]
- Magee P. T., Hopper A. K. Protein synthesis in relation to sporulation and meiosis in yeast. J Bacteriol. 1974 Sep;119(3):952–960. doi: 10.1128/jb.119.3.952-960.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matern H., Holzer H. Catabolite inactivation of the galactose uptake system in yeast. J Biol Chem. 1977 Sep 25;252(18):6399–6402. [PubMed] [Google Scholar]
- McCusker J. H., Haber J. E. Efficient sporulation of yeast in media buffered near pH6. J Bacteriol. 1977 Oct;132(1):180–185. doi: 10.1128/jb.132.1.180-185.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Piñon R. Effects of ammonium ions on sporulation of Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 15;105(2):367–378. doi: 10.1016/0014-4827(77)90134-3. [DOI] [PubMed] [Google Scholar]
- Polakis E. S., Bartley W. Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochem J. 1965 Oct;97(1):284–297. doi: 10.1042/bj0970284. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roth R., Halvorson H. O. Sporulation of yeast harvested during logarithmic growth. J Bacteriol. 1969 May;98(2):831–832. doi: 10.1128/jb.98.2.831-832.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simchen G. Are mitotic functions required in meiosis? Genetics. 1974 Apr;76(4):745–753. doi: 10.1093/genetics/76.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simchen G., Piñon R., Salts Y. Sporulation in Saccharomyces cerevisiae: premeiotic DNA synthesis, readiness and commitment. Exp Cell Res. 1972 Nov;75(1):207–218. doi: 10.1016/0014-4827(72)90538-1. [DOI] [PubMed] [Google Scholar]