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. 1982 Mar;149(3):1124–1128. doi: 10.1128/jb.149.3.1124-1128.1982

Isolation of mycoplasma membranes by dicyclohexylcarbodiimide-induced lysis.

M H Shirvan, S Rottem, Z Ne'eman, R Bittman
PMCID: PMC216504  PMID: 7061381

Abstract

A simple procedure was devised to prepared membranes from Mycoplasma gallisepticum cells. The cells were lysed in an isosmotic NaCl solution by dicyclohexylcarbodiimide, which blocks ATPase activity and interferes with the regulation of cell volume. The procedure can be used to isolate membranes of other osmotically resistant mycoplasmas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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