Abstract
Recently, two DNA polymerases (ScA and ScB) were isolated and characterized from Spiroplasma citri. We now have found a third DNA polymerase (ScC) not only in S. citri but also in the serologically related honeybee spiroplasma BC3 and the unrelated flower spiroplasma BNR1. Enzyme ScC is N-ethylmaleimide (NEM) sensitive. The three DNA polymerases from the honeybee spiroplasma seem to be similar to the respective enzymes of S. citri. However, whereas the NEM-resistant enzyme ScA from S. citri and that from the BC3 honeybee spiroplasma are retained on DEAE-cellulose and require 0.09 M KCl for elution, the NEM-resistant enzyme A from the flower spiroplasma BNR1 is not retained.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Charron A., Bebear C., Brun G., Yot P., Latrille J., Bove J. M. Separation and partial characterization of two deoxyribonucleic acid polymerases from Spiroplasma citri. J Bacteriol. 1979 Dec;140(3):763–768. doi: 10.1128/jb.140.3.763-768.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Litman R. M. A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. J Biol Chem. 1968 Dec 10;243(23):6222–6233. [PubMed] [Google Scholar]