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. 1982 Feb;149(2):542–547. doi: 10.1128/jb.149.2.542-547.1982

The herpes simplex virus thymidine kinase gene is not transcribed in Saccharomyces cerevisiae.

G B Kiss, R E Pearlman, K V Cornish, J D Friesen, V L Chan
PMCID: PMC216540  PMID: 6276360

Abstract

The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kinase coding sequence was detected.

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Selected References

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  1. Alwine J. C., Kemp D. J., Parker B. A., Reiser J., Renart J., Stark G. R., Wahl G. M. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979;68:220–242. doi: 10.1016/0076-6879(79)68017-5. [DOI] [PubMed] [Google Scholar]
  2. An G., Friesen J. D. Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacteriol. 1979 Nov;140(2):400–407. doi: 10.1128/jb.140.2.400-407.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bacchetti S., Graham F. L. Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1590–1594. doi: 10.1073/pnas.74.4.1590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beggs J. D., van den Berg J., van Ooyen A., Weissmann C. Abnormal expression of chromosomal rabbit beta-globin gene in Saccharomyces cerevisiae. Nature. 1980 Feb 28;283(5750):835–840. doi: 10.1038/283835a0. [DOI] [PubMed] [Google Scholar]
  5. Chevallier M. R., Aigle M. Qualitative detection of penicillinase produced by yeast strains carrying chimeric yeast-coli plasmids. FEBS Lett. 1979 Dec 1;108(1):179–180. doi: 10.1016/0014-5793(79)81204-1. [DOI] [PubMed] [Google Scholar]
  6. Chevallier M. R., Bloch J. C., Lacroute F. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene. 1980 Oct;11(1-2):11–19. doi: 10.1016/0378-1119(80)90082-7. [DOI] [PubMed] [Google Scholar]
  7. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  8. Cohen J. D., Eccleshall T. R., Needleman R. B., Federoff H., Buchferer B. A., Marmur J. Functional expression in yeast of the Escherichia coli plasmid gene coding for chloramphenicol acetyltransferase. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1078–1082. doi: 10.1073/pnas.77.2.1078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cryer D. R., Eccleshall R., Marmur J. Isolation of yeast DNA. Methods Cell Biol. 1975;12:39–44. doi: 10.1016/s0091-679x(08)60950-4. [DOI] [PubMed] [Google Scholar]
  10. Enquist L. W., Madden M. J., Schiop-Stanley P., Vande Woude G. F. Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector. Science. 1979 Feb 9;203(4380):541–544. doi: 10.1126/science.216076. [DOI] [PubMed] [Google Scholar]
  11. Enquist L. W., Vande Woude G. F., Wagner M., Smiley J. R., Summers W. C. Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of Herpes simplex type 1 virus. Gene. 1979 Nov;7(3-4):335–342. doi: 10.1016/0378-1119(79)90052-0. [DOI] [PubMed] [Google Scholar]
  12. Grivell A. R., Jackson J. F. Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid. J Gen Microbiol. 1968 Dec;54(2):307–317. doi: 10.1099/00221287-54-2-307. [DOI] [PubMed] [Google Scholar]
  13. Henikoff S., Tatchell K., Hall B. D., Nasmyth K. A. Isolation of a gene from Drosophila by complementation in yeast. Nature. 1981 Jan 1;289(5793):33–37. doi: 10.1038/289033a0. [DOI] [PubMed] [Google Scholar]
  14. Hinnen A., Hicks J. B., Fink G. R. Transformation of yeast. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929–1933. doi: 10.1073/pnas.75.4.1929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Klemperer H. G., Haynes G. R., Shedden W. I., Watson D. H. A virus-specific thymidine kinase in BHK-21 cells infected with herpes simplex virus. Virology. 1967 Jan;31(1):120–128. doi: 10.1016/0042-6822(67)90015-3. [DOI] [PubMed] [Google Scholar]
  16. Little J. G., Haynes R. H. Isolation and characterization of yeast mutants auxotrophic for 2'-deoxythymidine 5'-monophosphate. Mol Gen Genet. 1979 Jan 10;168(2):141–151. doi: 10.1007/BF00431440. [DOI] [PubMed] [Google Scholar]
  17. McKnight S. L., Gavis E. R. Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro. Nucleic Acids Res. 1980 Dec 20;8(24):5931–5948. doi: 10.1093/nar/8.24.5931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McKnight S. L. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. Nucleic Acids Res. 1980 Dec 20;8(24):5949–5964. doi: 10.1093/nar/8.24.5949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Panthier J. C., Fournier P., Heslot H., Rambach A. Sur l'expression d'un gène bactérien cloné dans la levure Saccharomyces cerevisiae. C R Seances Acad Sci D. 1979 Nov 5;289(11):773–775. [PubMed] [Google Scholar]
  20. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  21. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  22. Storms R. K., Holowachuck E. W., Friesen J. D. Genetic complementation of the Saccharomyces cerevisiae leu2 gene by the Escherichia coli leuB gene. Mol Cell Biol. 1981 Sep;1(9):836–842. doi: 10.1128/mcb.1.9.836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Storms R. K., McNeil J. B., Khandekar P. S., An G., Parker J., Friesen J. D. Chimeric plasmids for cloning of deoxyribonucleic acid sequences in Saccharomyces cerevisiae. J Bacteriol. 1979 Oct;140(1):73–82. doi: 10.1128/jb.140.1.73-82.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Warner J. R., Gorenstein C. The synthesis of eucaryotic ribosomal proteins in vitro. Cell. 1977 May;11(1):201–212. doi: 10.1016/0092-8674(77)90331-2. [DOI] [PubMed] [Google Scholar]
  25. Wigler M., Pellicer A., Silverstein S., Axel R. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell. 1978 Jul;14(3):725–731. doi: 10.1016/0092-8674(78)90254-4. [DOI] [PubMed] [Google Scholar]

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