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. 1982 Feb;149(2):654–661. doi: 10.1128/jb.149.2.654-661.1982

Isolation and characterization of Pseudomonas aeruginosa R' plasmids constructed by interspecific mating.

A F Morgan
PMCID: PMC216555  PMID: 6799492

Abstract

Plasmid R68.45 was used to construct R' plasmids carrying a maximum of 4 to 5 map minutes of the Pseudomonas aeruginosa PAO chromosome by interspecific mating, using P. putida PPN as the recipient. These R' plasmids were used to determine the map location of the amiE locus and to identify tentatively a number of P. putida auxotrophic mutations. Some of these R' plasmids could not be maintained in recombination-deficient P. aeruginosa strains.

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Selected References

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