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. 1982 Feb;149(2):789–792. doi: 10.1128/jb.149.2.789-792.1982

Secretion and processing of ribose-binding protein in Escherichia coli.

J L Garwin, J Beckwith
PMCID: PMC216576  PMID: 7035438

Abstract

The periplasmic D-ribose-binding protein of Escherichia coli K-12 is made initially as a larger precursor form. This precursor was observed in wild-type cells and more stably in cells inhibited for protein secretion. The precursor could be processed to the mature D-ribose-binding protein either co-or posttranslationally. The secretion pathway of the D-ribose-binding protein and that of the maltose-binding secretion have many characteristics in common.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beacham I. R. Periplasmic enzymes in gram-negative bacteria. Int J Biochem. 1979;10(11):877–883. doi: 10.1016/0020-711x(79)90117-4. [DOI] [PubMed] [Google Scholar]
  2. Chamberlain J. P. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979 Sep 15;98(1):132–135. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
  3. Galloway D. R., Furlong C. E. The role of ribose-binding protein in transport and chemotaxis in Escherichia coli K12. Arch Biochem Biophys. 1977 Dec;184(2):496–504. doi: 10.1016/0003-9861(77)90459-3. [DOI] [PubMed] [Google Scholar]
  4. Ito K., Bassford P. J., Jr, Beckwith J. Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins? Cell. 1981 Jun;24(3):707–717. doi: 10.1016/0092-8674(81)90097-0. [DOI] [PubMed] [Google Scholar]
  5. Koshland D., Botstein D. Secretion of beta-lactamase requires the carboxy end of the protein. Cell. 1980 Jul;20(3):749–760. doi: 10.1016/0092-8674(80)90321-9. [DOI] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Rock C. O., Cronan J. E., Jr Re-evaluation of the solution structure of acyl carrier protein. J Biol Chem. 1979 Oct 10;254(19):9778–9785. [PubMed] [Google Scholar]
  8. Tjian R., Stinchcomb D., Losick R. Antibody directed against Bacillus subtilis rho factor purified by sodium dodecyl sulfate slab gel electrophoresis. Effect on transcription by RNA polymerase in crude extracts of vegetative and sporulating cells. J Biol Chem. 1975 Nov 25;250(22):8824–8828. [PubMed] [Google Scholar]
  9. Willis R. C., Furlong C. E. Purification and properties of a ribose-binding protein from Escherichia coli. J Biol Chem. 1974 Nov 10;249(21):6926–6929. [PubMed] [Google Scholar]

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