Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Jan;149(1):54–58. doi: 10.1128/jb.149.1.54-58.1982

Glycolipids from some extreme thermophilic bacteria belonging to the genus Thermus.

R A Pask-Hughes, N Shaw
PMCID: PMC216591  PMID: 7054151

Abstract

The lipids of Thermus aquaticus YT1, Thermus thermophilus HB8, Thermus sp. strains H and J (from Icelandic hot springs), and Thermus sp. strain NH (from domestic hot water) have been investigated. Each strain contained two major components, a glycolipid and a glycophospholipid, which have been isolated and analyzed. All of the strains contained as the principal component (41 to 57% of total lipid) a diacyldiglycosyl-(N-acyl)glycosaminylglucosylglycerol, but the five glycolipids differed in carbohydrate composition. The glycophospholipid appeared to be identical in each strain and contained an N-acylglucosamine residue. The principal fatty acids were C15 and C17 branched-chain compounds. This unique polar lipid composition should be of value in the classification of other thermophiles in the genus Thermus. The exceptionally high carbohydrate content of the lipids of these extreme thermophiles may be of significance in relation to the molecular basis of thermophily.

Full text

PDF
54

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  2. Langworthy T. A., Mayberry W. R., Smith P. F. A sulfonolipid and novel glucosamidyl glycolipids from the extreme thermoacidophile Bacillus acidocaldarius. Biochim Biophys Acta. 1976 Jun 22;431(3):550–569. doi: 10.1016/0005-2760(76)90220-4. [DOI] [PubMed] [Google Scholar]
  3. Langworthy T. A., Mayberry W. R., Smith P. F. Long-chain glycerol diether and polyol dialkyl glycerol triether lipids of Sulfolobus acidocaldarius. J Bacteriol. 1974 Jul;119(1):106–116. doi: 10.1128/jb.119.1.106-116.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Langworthy T. A., Smith P. F., Mayberry W. R. Lipids of Thermoplasma acidophilum. J Bacteriol. 1972 Dec;112(3):1193–1200. doi: 10.1128/jb.112.3.1193-1200.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Oshima M., Yamakawa T. Chemical structure of a novel glycolipid from an extreme thermophile, Flavobacterium thermophilum. Biochemistry. 1974 Mar 12;13(6):1140–1146. doi: 10.1021/bi00703a014. [DOI] [PubMed] [Google Scholar]
  6. Pask-Hughes R., Williams R. A. Extremely thermophilic gram-negative bacteria from hot tap water. J Gen Microbiol. 1975 Jun;88(2):321–328. doi: 10.1099/00221287-88-2-321. [DOI] [PubMed] [Google Scholar]
  7. Ray P. H., White D. C., Brock T. D. Effect of growth temperature on the lipid composition of Thermus aquaticus. J Bacteriol. 1971 Oct;108(1):227–235. doi: 10.1128/jb.108.1.227-235.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ray P. H., White D. C., Brock T. D. Effect of temperature on the fatty acid composition of Thermus aquaticus. J Bacteriol. 1971 Apr;106(1):25–30. doi: 10.1128/jb.106.1.25-30.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shaw N. Lipid composition as a guide to the classification of bacteria. Adv Appl Microbiol. 1974;17(0):63–108. doi: 10.1016/s0065-2164(08)70555-0. [DOI] [PubMed] [Google Scholar]
  10. Shaw N., Stead D. Lipid composition of some species of Arthrobacter. J Bacteriol. 1971 Jul;107(1):130–133. doi: 10.1128/jb.107.1.130-133.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shaw N. The detection of lipids on thin-layer chromatograms with the periodate-Schiff reagents. Biochim Biophys Acta. 1968 Oct 22;164(2):435–436. doi: 10.1016/0005-2760(68)90171-9. [DOI] [PubMed] [Google Scholar]
  12. Tsuji A., Kinoshita T., Hoshino M. Analytical chemical studies on amino sugars. II. Determination of hexosamines using 3-methyl-2-benzothiazolone hydrazone hydrochloride. Chem Pharm Bull (Tokyo) 1969 Jul;17(7):1505–1510. doi: 10.1248/cpb.17.1505. [DOI] [PubMed] [Google Scholar]
  13. VORBECK M. L., MARINETTI G. V. SEPARATION OF GLYCOSYL DIGLYCERIDES FROM PHOSPHATIDES USING SILICIC ACID COLUMN CHROMATOGRAPHY. J Lipid Res. 1965 Jan;6:3–6. [PubMed] [Google Scholar]
  14. WELLS M. A., DITTMER J. C. THE USE OF SEPHADEX FOR THE REMOVAL OF NONLIPID CONTAMINANTS FROM LIPID EXTRACTS. Biochemistry. 1963 Nov-Dec;2:1259–1263. doi: 10.1021/bi00906a015. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES