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. 1982 Jan;149(1):320–328. doi: 10.1128/jb.149.1.320-328.1982

Specific antisera and immunological procedures for characterization of methanogenic bacteria.

E Conway de Macario, A J Macario, M J Wolin
PMCID: PMC216625  PMID: 6172417

Abstract

Specific antisera were raised in rabbits to 19 methanogenic bacteria representing the species available in pure culture at the present time. The antisera were characterized, labeled, and organized in a bank to serve as a source of material for preparation of antibody probes and thus provide standardized reagents for immunological analysis of methanogens. An indirect immunofluorescence procedure was standardized for optimal staining of homologous and heterologous bacterial strains. Two immunoenzymatic assays were developed: (i) a simple slide assay, useful for rapid antibody detection in small samples, antibody titrations, and disclosure of cross-reactions among methanogens, and (ii) a quantitative method. The latter is useful for quantification of antigenic relatedness. Procedural details were developed to obtain optimal bacterial preparations for use as immunogens to raise antibodies in vivo, and as antigens for antibody assay in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Balch W. E., Wolfe R. S. Specificity and biological distribution of coenzyme M (2-mercaptoethanesulfonic acid). J Bacteriol. 1979 Jan;137(1):256–263. doi: 10.1128/jb.137.1.256-263.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cheeseman P., Toms-Wood A., Wolfe R. S. Isolation and properties of a fluorescent compound, factor 420 , from Methanobacterium strain M.o.H. J Bacteriol. 1972 Oct;112(1):527–531. doi: 10.1128/jb.112.1.527-531.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen M., Wolin M. J. Influence of CH4 production by Methanobacterium ruminantium on the fermentation of glucose and lactate by Selenomonas ruminantium. Appl Environ Microbiol. 1977 Dec;34(6):756–759. doi: 10.1128/aem.34.6.756-759.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Conway de Macario E., Macario A. J. Molecular mechanisms of the immunologic activation of a defective Escherichia coli beta-D-galactosidase--I. Immunochemistry of rabbit antibodies at various times during the immune response and their interaction with the enzyme. Immunochemistry. 1978 Sep;15(9):681–685. doi: 10.1016/0161-5890(78)90042-1. [DOI] [PubMed] [Google Scholar]
  6. Conway de Macario E., Wolin M. J., Macario A. J. Antibody analysis of relationships among methanogenic bacteria. J Bacteriol. 1982 Jan;149(1):316–319. doi: 10.1128/jb.149.1.316-319.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Conway de Macario E., Wolin M. J., Macario A. J. Immunology of archaebacteria that produce methane gas. Science. 1981 Oct 2;214(4516):74–75. doi: 10.1126/science.6792708. [DOI] [PubMed] [Google Scholar]
  8. Diekert G., Klee B., Thauer R. K. Nickel, a component of factor F430 from Methanobacterium thermoautotrophicum. Arch Microbiol. 1980 Jan;124(1):103–106. doi: 10.1007/BF00407036. [DOI] [PubMed] [Google Scholar]
  9. Fox G. E., Magrum L. J., Balch W. E., Wolfe R. S., Woese C. R. Classification of methanogenic bacteria by 16S ribosomal RNA characterization. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4537–4541. doi: 10.1073/pnas.74.10.4537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gunsalus R. P., Romesser J. A., Wolfe R. S. Preparation of coenzyme M analogues and their activity in the methyl coenzyme M reductase system of Methanobacterium thermoautotrophicum. Biochemistry. 1978 Jun 13;17(12):2374–2377. doi: 10.1021/bi00605a019. [DOI] [PubMed] [Google Scholar]
  11. Kandler O., Hippe H. Lack of peptidoglycan in the cell walls of Methanosarcina barkeri. Arch Microbiol. 1977 May 13;113(1-2):57–60. doi: 10.1007/BF00428580. [DOI] [PubMed] [Google Scholar]
  12. Kandler O., König H. Chemical composition of the peptidoglycan-free cell walls of methanogenic bacteria. Arch Microbiol. 1978 Aug 1;118(2):141–152. doi: 10.1007/BF00415722. [DOI] [PubMed] [Google Scholar]
  13. König H., Kandler O. 2-amino-2-deoxytaluronic acid and 2-amino-2-deoxyglucose from the pseudomurein of Methanobacterium termoautotrophicum possess the L- and D-configurations, respectively. Hoppe Seylers Z Physiol Chem. 1980;361(6):981–983. [PubMed] [Google Scholar]
  14. König H., Kandler O. The amino acid sequence of the peptide moiety of the pseudomurein from Methanobacterium thermoautotrophicum. Arch Microbiol. 1979 Jun;121(3):271–275. doi: 10.1007/BF00425067. [DOI] [PubMed] [Google Scholar]
  15. Mah R. A., Smith M. R., Baresi L. Studies on an acetate-fermenting strain of Methanosarcina. Appl Environ Microbiol. 1978 Jun;35(6):1174–1184. doi: 10.1128/aem.35.6.1174-1184.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McBride B. C., Wolfe R. S. A new coenzyme of methyl transfer, coenzyme M. Biochemistry. 1971 Jun 8;10(12):2317–2324. doi: 10.1021/bi00788a022. [DOI] [PubMed] [Google Scholar]
  17. Miller T. L., Wolin M. J. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol. 1974 May;27(5):985–987. doi: 10.1128/am.27.5.985-987.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miller T. L., Wolin M. J. Fermentation by the human large intestine microbial community in an in vitro semicontinuous culture system. Appl Environ Microbiol. 1981 Sep;42(3):400–407. doi: 10.1128/aem.42.3.400-407.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shapiro S., Wolfe R. S. Methyl-coenzyme M, an intermediate in methanogenic dissimilation of C1 compounds by Methanosarcina barkeri. J Bacteriol. 1980 Feb;141(2):728–734. doi: 10.1128/jb.141.2.728-734.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strayer R. F., Tiedje J. M. Application of the fluorescent-antibody technique to the study of a methanogenic bacterium in lake sediments. Appl Environ Microbiol. 1978 Jan;35(1):192–198. doi: 10.1128/aem.35.1.192-198.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Taylor C. D., McBride B. C., Wolfe R. S., Bryant M. P. Coenzyme M, essential for growth of a rumen strain of Methanobacterium ruminantium. J Bacteriol. 1974 Nov;120(2):974–975. doi: 10.1128/jb.120.2.974-975.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tzeng S. F., Wolfe R. S., Bryant M. P. Factor 420-dependent pyridine nucleotide-linked hydrogenase system of Methanobacterium ruminantium. J Bacteriol. 1975 Jan;121(1):184–191. doi: 10.1128/jb.121.1.184-191.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tzing S. F., Bryant M. P., Wolfe R. S. Factor 420-dependent pyridine nucleotide-linked formate metabolism of Methanobacterium ruminantium. J Bacteriol. 1975 Jan;121(1):192–196. doi: 10.1128/jb.121.1.192-196.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ward T. E., Frea J. I. Sediment distribution of methanogenic bacteria in lake erie and cleveland harbor. Appl Environ Microbiol. 1980 Mar;39(3):597–603. doi: 10.1128/aem.39.3.597-603.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Whitman W. B., Wolfe R. S. Presence of nickel in factor F430 from Methanobacterium bryantii. Biochem Biophys Res Commun. 1980 Feb 27;92(4):1196–1201. doi: 10.1016/0006-291x(80)90413-1. [DOI] [PubMed] [Google Scholar]
  26. Woese C. R. A comment on methanogenic bacteria and the primitive ecology. J Mol Evol. 1977 Aug 5;9(4):369–371. doi: 10.1007/BF01796101. [DOI] [PubMed] [Google Scholar]
  27. Woese C. R., Fox G. E. Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5088–5090. doi: 10.1073/pnas.74.11.5088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Woese C. R., Magrum L. J., Fox G. E. Archaebacteria. J Mol Evol. 1978 Aug 2;11(3):245–251. doi: 10.1007/BF01734485. [DOI] [PubMed] [Google Scholar]
  29. Zeikus J. G. The biology of methanogenic bacteria. Bacteriol Rev. 1977 Jun;41(2):514–541. doi: 10.1128/br.41.2.514-541.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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