Abstract
The uptake of methylamine as the sole nitrogen, but not carbon, source by Pseudomonas sp. strain MA was investigated. Under these growth conditions, a high-affinity, low-capacity uptake system was present having a Km of 16 microM and Vmax of 2 nmol.min-.mg (dry weight) of cells that was competitively inhibited by ammonium chloride. The transport system was induced by growth on succinate with methylamine as the sole nitrogen source.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bellion E., Hersh L. B. Methylamine metabolism in a pseudomonas species. Arch Biochem Biophys. 1972 Nov;153(1):368–374. doi: 10.1016/0003-9861(72)90457-2. [DOI] [PubMed] [Google Scholar]
- Bellion E., Khan M. Y., Romano M. J. Transport of methylamine by Pseudomonas sp. MA. J Bacteriol. 1980 Jun;142(3):786–790. doi: 10.1128/jb.142.3.786-790.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bellion E., Kim Y. S. Catabolite repression of isocitrate lyase in methylamine-grown Pseudomonas MA. Effect of carbon and nitrogen sources. Biochim Biophys Acta. 1978 Jul 17;541(4):425–434. doi: 10.1016/0304-4165(78)90152-6. [DOI] [PubMed] [Google Scholar]
- Hackette S. L., Skye G. E., Burton C., Segel I. H. Characterization of an ammonium transport system in filamentous fungi with methylammonium-14C as the substrate. J Biol Chem. 1970 Sep 10;245(17):4241–4250. [PubMed] [Google Scholar]
- Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
- Pateman J. A., Dunn E., Kinghorn J. R., Forbes E. C. The transport of ammonium and methylammonium in wild type and mutant cells of Aspergillus nidulans. Mol Gen Genet. 1974;133(3):225–236. doi: 10.1007/BF00267672. [DOI] [PubMed] [Google Scholar]
- Roon R. J., Even H. L., Dunlop P., Larimore F. L. Methylamine and ammonia transport in Saccharomyces cerevisiae. J Bacteriol. 1975 May;122(2):502–509. doi: 10.1128/jb.122.2.502-509.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roon R. J., Levy J. S., Larimore F. Negative interactions between amino acid and methylamine/ammonia transport systems of Saccharomyces cerevisiae. J Biol Chem. 1977 Jun 10;252(11):3599–3604. [PubMed] [Google Scholar]
- Shaw W. V., Tsai L., Stadtman E. R. The enzymatic synthesis of N-methylglutamic acid. J Biol Chem. 1966 Feb 25;241(4):935–945. [PubMed] [Google Scholar]
- Stevenson R., Silver S. Methylammonium uptake by Escherichia coli: evidence for a bacterial NH4+ transport system. Biochem Biophys Res Commun. 1977 Apr 25;75(4):1133–1139. doi: 10.1016/0006-291x(77)91501-7. [DOI] [PubMed] [Google Scholar]