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. 1982 Jan;149(1):407–410. doi: 10.1128/jb.149.1.407-410.1982

Amino-terminal sequence of the Tn3 transposase protein.

M D Ditto, J Chou, M W Hunkapiller, M A Fennewald, S P Gerrard, L E Hood, S N Cohen, M J Casadaban
PMCID: PMC216644  PMID: 6274848

Abstract

The amino-terminal sequence of the Tn3 transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-beta-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-beta-galactosidase gene fusions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur A., Sherratt D. Dissection of the transposition process: a transposon-encoded site-specific recombination system. Mol Gen Genet. 1979 Oct 1;175(3):267–274. doi: 10.1007/BF00397226. [DOI] [PubMed] [Google Scholar]
  2. Casadaban M. J., Chou J., Cohen S. N. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980 Aug;143(2):971–980. doi: 10.1128/jb.143.2.971-980.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Casadaban M. J., Chou J., Lemaux P., Tu C. P., Cohen S. N. Tn3: transposition and control. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):269–273. doi: 10.1101/sqb.1981.045.01.039. [DOI] [PubMed] [Google Scholar]
  4. Chou J., Casadaban M. J., Lemaux P. G., Cohen S. N. Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4020–4024. doi: 10.1073/pnas.76.8.4020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chou J., Lemaux P. G., Casadaban M. J., Cohen S. N. Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product. Nature. 1979 Dec 20;282(5741):801–806. doi: 10.1038/282801a0. [DOI] [PubMed] [Google Scholar]
  6. Fennewald M. A., Gerrard S. P., Chou J., Casadaban M. J., Cozzarelli N. R. Purification of the Tn3 transposase and analysis of its binding to DNA. J Biol Chem. 1981 May 25;256(10):4687–4690. [PubMed] [Google Scholar]
  7. Gill R. E., Heffron F., Falkow S. Identification of the protein encoded by the transposable element Tn3 which is required for its transposition. Nature. 1979 Dec 20;282(5741):797–801. doi: 10.1038/282797a0. [DOI] [PubMed] [Google Scholar]
  8. Gill R., Heffron F., Dougan G., Falkow S. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3. J Bacteriol. 1978 Nov;136(2):742–756. doi: 10.1128/jb.136.2.742-756.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heffron F., McCarthy B. J., Ohtsubo H., Ohtsubo E. DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell. 1979 Dec;18(4):1153–1163. doi: 10.1016/0092-8674(79)90228-9. [DOI] [PubMed] [Google Scholar]
  10. Heffron F., So M., McCarthy B. J. In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6012–6016. doi: 10.1073/pnas.75.12.6012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hunkapiller M. W., Hood L. E. New protein sequenator with increased sensitivity. Science. 1980 Feb 1;207(4430):523–525. doi: 10.1126/science.7352258. [DOI] [PubMed] [Google Scholar]
  12. Ikeda J. E., Yudelevich A., Hurwitz J. Isolation and characterization of the protein coded by gene A of bacteriophage phiX174 DNA. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2669–2673. doi: 10.1073/pnas.73.8.2669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Johnson R. C., Reznikoff W. S. Localization of the Tn5 transposase promoter using the cycling reaction of RNA polymerase. Nucleic Acids Res. 1981 Apr 24;9(8):1873–1883. doi: 10.1093/nar/9.8.1873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Reed R. R. Resolution of cointegrates between transposons gamma delta and Tn3 defines the recombination site. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3428–3432. doi: 10.1073/pnas.78.6.3428. [DOI] [PMC free article] [PubMed] [Google Scholar]

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