Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Nov;140(2):311–319. doi: 10.1128/jb.140.2.311-319.1979

Inversion in the lactose region of Escherichia coli K-12.

D J Savic
PMCID: PMC216651  PMID: 387729

Abstract

A spontaneous mutant of Escherichia coli K-12, strain SY99, with an inversion in the lactose region was isolated and partially characterized. The inversion was detected due to inverse chromosomal conjugational transfer after introduction of an F42 (F'lac) episome. The termini of the inversion are between proAB and lac on one side and lac and proC on the other. The inverse conjugational transfer in SY99 did not appear to be absolute but was always accompanied by a residual "normal" counterclockwise mobilization. This residual transfer was further shown to be caused by the intrinsic instability of this region (at least in the line W3110). The possible involvement of IS3 elements flanking the lactose operon is discussed.

Full text

PDF
311

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berg C. M. Auxotroph accumulation in deoxyribonucleic acid polymeraseless strains of Escherichia coli K-12. J Bacteriol. 1971 Jun;106(3):797–801. doi: 10.1128/jb.106.3.797-801.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berg C. M., Curtiss R., 3rd Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics. 1967 Jul;56(3):503–525. doi: 10.1093/genetics/56.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bergquist P. L., Jamieson A. F. Genetic inversion in the formation of an Hfr strain from a temperature-sensitive F' gal strain. J Bacteriol. 1977 Jan;129(1):282–290. doi: 10.1128/jb.129.1.282-290.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Besemer J., Kubai D. F. Isolation and characterization of phi80dgal transducing phages that carry gal operator-promoter insertion mutations. Mol Gen Genet. 1976 Oct 18;148(1):79–82. doi: 10.1007/BF00268548. [DOI] [PubMed] [Google Scholar]
  7. CUZIN F., JACOB F. [Reversible integration of the F' sexual episome in Escherichia coli K 12]. C R Hebd Seances Acad Sci. 1963 Jul 17;257:795–797. [PubMed] [Google Scholar]
  8. Calendar R., Lindahl G., Marsh M., Sunshine M. Temperature-inducible mutants of P2 phage. Virology. 1972 Jan;47(1):68–75. doi: 10.1016/0042-6822(72)90240-1. [DOI] [PubMed] [Google Scholar]
  9. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dewitt S K, Adelberg E A. The Occurrence of a Genetic Transposition in a Strain of Escherichia Coli. Genetics. 1962 May;47(5):577–585. doi: 10.1093/genetics/47.5.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hu S., Ohtsubo E., Davidson N. Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes. J Bacteriol. 1975 May;122(2):749–763. doi: 10.1128/jb.122.2.749-763.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JACOB F., ADELBERG E. A. Transfert de caractéres génétiques par incorporation au facteur sexuel d'Escherichia coli. C R Hebd Seances Acad Sci. 1959 Jul 6;249(1):189–191. [PubMed] [Google Scholar]
  13. Kopecko D. J., Cohen S. N. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1373–1377. doi: 10.1073/pnas.72.4.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuempel P. L., Duerr S. A., Maglothin P. D. Chromosome replication in an Escherichia coli dnaA mutant integratively suppressed by prophage P2. J Bacteriol. 1978 Jun;134(3):902–912. doi: 10.1128/jb.134.3.902-912.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lee H. J., Otsubo E., Deonier R. C., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv+ Deletion mutants of F14. J Mol Biol. 1974 Nov 15;89(4):585–597. doi: 10.1016/0022-2836(74)90037-0. [DOI] [PubMed] [Google Scholar]
  16. Lindahl G., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli: replication of the bacterial chromosome under control of prophage P2. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2407–2411. doi: 10.1073/pnas.68.10.2407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lindahl G. Multiple recombination mechanisms in bacteriophage P2. Virology. 1969 Dec;39(4):861–866. doi: 10.1016/0042-6822(69)90022-1. [DOI] [PubMed] [Google Scholar]
  18. Lindqvist B. H., Six E. W. Replication of bacteriophage P4 DNA in a nonlysogenic host. Virology. 1971 Jan;43(1):1–7. doi: 10.1016/0042-6822(71)90218-2. [DOI] [PubMed] [Google Scholar]
  19. Low B. Low recombination frequency for markers very near the origin in conjugation in E. coli. Genet Res. 1965 Nov;6(3):469–473. doi: 10.1017/s0016672300004341. [DOI] [PubMed] [Google Scholar]
  20. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. PITTARD J., LOUTIT J. S., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. I. DELAY IN INITIATION OF CHROMOSOME TRANSFER. J Bacteriol. 1963 Jun;85:1394–1401. doi: 10.1128/jb.85.6.1394-1401.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pittard J., Walker E. M. Conjugation in Escherichia coli: recombination events in terminal regions of transferred deoxyribonucleic acid. J Bacteriol. 1967 Nov;94(5):1656–1663. doi: 10.1128/jb.94.5.1656-1663.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
  24. Starlinger P., Saedler H. IS-elements in microorganisms. Curr Top Microbiol Immunol. 1976;75:111–152. doi: 10.1007/978-3-642-66530-1_4. [DOI] [PubMed] [Google Scholar]
  25. Sunshine M. G., Kelly B. Extent of host deletions associated with bacteriophage P2-mediated eduction. J Bacteriol. 1971 Nov;108(2):695–704. doi: 10.1128/jb.108.2.695-704.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  27. Youngs D. A., Smith K. C. Evidence for the control by exrA and polA genes of two branches of the uvr gene-dependent excision repair pathway in Escherichia coli K-12. J Bacteriol. 1973 Oct;116(1):175–182. doi: 10.1128/jb.116.1.175-182.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES