Abstract
A new batch calorimeter was used to study the aerobic growth of Escherichia coli in a minimal containing a growth-limiting concentration of succinic acid. The shape of the thermograms obtained was simple when the energy source was the only growth-limiting factor and showed a stationary phase when the oxygen transfer was limiting. The experimentally determined value of the heat production during growth was found to be significantly lower than the heat of combustion of the succinic acid corrected for the assimilated-substrate fraction. An interpretation has been given to explain the difference between the experimental and the theoretical values.
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Selected References
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- Belaich A., Belaich J. P. Microcalorimetric study of the anaerobic growth of Escherichia coli: growth thermograms in a synthetic medium. J Bacteriol. 1976 Jan;125(1):14–18. doi: 10.1128/jb.125.1.14-18.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Belaich A., Belaich J. P. Microcalorimetric study of the anaerobic growth of Escherichia coli: measurements of the affinity of whole cells for various energy substrates. J Bacteriol. 1976 Jan;125(1):19–24. doi: 10.1128/jb.125.1.19-24.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Belaich J. P., Senez J. C., Murgier M. Microcalorimetric study of glucose permeation in microbial cells. J Bacteriol. 1968 May;95(5):1750–1757. doi: 10.1128/jb.95.5.1750-1757.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Few G. A., Yau A. O., Prichard F. E., James A. M. A microcalorimetric study of the growth of Klebsiella aerogenes in simple salts/glucose media. Microbios. 1976;16(63):37–48. [PubMed] [Google Scholar]
- Monk P. R. Aerobic growth thermograms of Streptococcus lactis obtained with a complex medium containing glucose. J Bacteriol. 1978 Aug;135(2):373–378. doi: 10.1128/jb.135.2.373-378.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murgier M., Belaich J. P. Microcalorimetric determination of the affinity of Saccharomyces cerevisiae for some carbohydrate growth substrates. J Bacteriol. 1971 Feb;105(2):573–579. doi: 10.1128/jb.105.2.573-579.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ONOUE K., KITAGAWA M., YAMAMURA Y. Chemical studies on cellular components of Bordetella pertussis. I. Purification and properties of agglutinogen. J Bacteriol. 1961 Nov;82:648–656. doi: 10.1128/jb.82.5.648-656.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wadsö I. A system of micro-calorimeters and its use in biochemistry and biology. Biochem Soc Trans. 1976;4(4):561–565. doi: 10.1042/bst0040561a. [DOI] [PubMed] [Google Scholar]
