Abstract
A cytoplasmic component which inhibited the activation of chitin synthetase was studied in the dimorphic fungus Candida albicans. The inhibitor was found to be heat stable and trypsin sensitive and was only effective when incubated with a vacuolar protease, an activator of chitin synthetase, before the activation of chitin synthetase. In addition, the particulate chitin synthetase from the yeast form of C. albicans was solubilized by a sodium cholate-digitonin extraction and subsequently was purified approximately 30-fold by Sepharose column chromatography and Amicon XM 100 filtration. Activity of the soluble enzyme was increased by the addition of trypsin or phosphatidyl serine. The molecular weight of the enzyme was estimated to be 400,000.
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- Braun P. C., Calderone R. A. Chitin synthesis in Candida albicans: comparison of yeast and hyphal forms. J Bacteriol. 1978 Mar;133(3):1472–1477. doi: 10.1128/jb.133.3.1472-1477.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cabib E., Farkas V. The control of morphogenesis: an enzymatic mechanism for the initiation of septum formation in yeast. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2052–2056. doi: 10.1073/pnas.68.9.2052. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cabib E., Keller F. A. Chitin and yeast budding. Allosteric inhibition of chitin synthetase by a heat-stable protein from yeast. J Biol Chem. 1971 Jan 10;246(1):167–173. [PubMed] [Google Scholar]
- Cabib E. Molecular aspects of yeast morphogenesis. Annu Rev Microbiol. 1975;29:191–214. doi: 10.1146/annurev.mi.29.100175.001203. [DOI] [PubMed] [Google Scholar]
- Duran A., Cabib E. Solubilization and partial purification of yeast chitin synthetase. Confirmation of the zymogenic nature of the enzyme. J Biol Chem. 1978 Jun 25;253(12):4419–4425. [PubMed] [Google Scholar]
- Huang K. P., Cabib E. Yeast glycogen synthetase in the glucose 6-phosphate independent form: a case of cold lability without major changes in molecular size. Biochim Biophys Acta. 1973 Apr 12;302(2):240–248. doi: 10.1016/0005-2744(73)90153-8. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- McMurrough I., Bartnicki-Garcia S. Inhibition and activation of chitin synthesis by Mucor rouxii cell extracts. Arch Biochem Biophys. 1973 Oct;158(2):812–816. doi: 10.1016/0003-9861(73)90576-6. [DOI] [PubMed] [Google Scholar]
- Ulane R. E., Cabib E. The activating system of chitin synthetase from Saccharomyces cerevisiae. Purification and properties of an inhibitor of the activating factor. J Biol Chem. 1974 Jun 10;249(11):3418–3422. [PubMed] [Google Scholar]