Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Nov;140(2):734–737. doi: 10.1128/jb.140.2.734-737.1979

Selection for Escherichia coli mutants with proteins missing from the ribosome.

E R Dabbs
PMCID: PMC216704  PMID: 387748

Abstract

Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing.

Full text

PDF
734

Images in this article

735Image
on p.735
736Image
on p.736

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bollen A., Davies J., Ozaki M., Mizushima S. Ribosomal Protein Conferring Sensitivity to the Antibiotic Spectinomycin in Escherichia coli. Science. 1969 Jul 4;165(3888):85–86. doi: 10.1126/science.165.3888.85. [DOI] [PubMed] [Google Scholar]
  2. Dabbs E. R. A spectinomycin dependent mutant of Escherichia coli. Mol Gen Genet. 1977 Mar 16;151(3):261–267. doi: 10.1007/BF00268789. [DOI] [PubMed] [Google Scholar]
  3. Dabbs E. R. Kasugamycin-dependent mutants of Escherichia coli. J Bacteriol. 1978 Dec;136(3):994–1001. doi: 10.1128/jb.136.3.994-1001.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dabbs E. R. Mutational alterations in 50 proteins of the Escherichia coli ribosome. Mol Gen Genet. 1978 Sep 20;165(1):73–78. doi: 10.1007/BF00270378. [DOI] [PubMed] [Google Scholar]
  5. Dabbs E. R., Wittman H. G. A strain of Escherichia coli which gives rise to mutations in a large number of ribosomal proteins. Mol Gen Genet. 1976 Dec 22;149(3):303–309. doi: 10.1007/BF00268532. [DOI] [PubMed] [Google Scholar]
  6. Hasenbank R., Guthrie C., Stöffler G., Wittmann H. G., Rosen L., Apirion D. Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Mol Gen Genet. 1973 Dec 14;127(1):1–18. doi: 10.1007/BF00267778. [DOI] [PubMed] [Google Scholar]
  7. Isono S., Isono K. Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins in Escherichia coli. Mol Gen Genet. 1978 Sep 20;165(1):15–20. doi: 10.1007/BF00270371. [DOI] [PubMed] [Google Scholar]
  8. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
  9. Parker J., Watson R. J., Friesen J. D. A relaxed mutant with an altered ribosomal protein L11. Mol Gen Genet. 1976 Feb 27;144(1):111–114. doi: 10.1007/BF00277313. [DOI] [PubMed] [Google Scholar]
  10. Sparling P. F., Blackman E. Mutation to erythromycin dependence in Escherichia coli K-12. J Bacteriol. 1973 Oct;116(1):74–83. doi: 10.1128/jb.116.1.74-83.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Traub P., Nomura M. Streptomycin resistance mutation in Escherichia coli: altered ribosomal protein. Science. 1968 Apr 12;160(3824):198–199. doi: 10.1126/science.160.3824.198. [DOI] [PubMed] [Google Scholar]
  12. Wittmann H. G., Stöffler G., Apirion D., Rosen L., Tanaka K., Tamaki M., Takata R., Dekio S., Otaka E. Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins. Mol Gen Genet. 1973 Dec 20;127(2):175–189. doi: 10.1007/BF00333665. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES