Abstract
The structural relationship between the transfer ribonucleic acid (tRNA) and the ribosomal RNA (rRNA) genes of Bacillus subtilis has been studied by restriction endonuclease analysis of total chromosomal deoxyribonucleic acid (DNA) and characterization of DNA fragments cloned in Escherichia coli. The DNA sequences encoding rRNA and tRNA were assayed by hybridization to radioactive RNA. The results support the conclusion that the tRNA genes are interspersed between and closely linked to the rRNA genes of B. subtilis. They probably do not appear between the 16S and 23S rRNA genes as in E. coli.
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Selected References
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- Chow L. T., Davidson N. Electron microscope mapping of the distribution of ribosomal genes of the Bacillus subtilis chromosome. J Mol Biol. 1973 Apr 5;75(2):265–279. doi: 10.1016/0022-2836(73)90020-x. [DOI] [PubMed] [Google Scholar]
- Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. J Biol Chem. 1973 Jul 25;248(14):5024–5032. [PubMed] [Google Scholar]
- Moran C. P., Jr, Bott K. F. Restriction enzyme analysis of Bacillus subtilis ribosomal ribonucleic acid genes. J Bacteriol. 1979 Oct;140(1):99–105. doi: 10.1128/jb.140.1.99-105.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nomura M. Organization of bacterial genes for ribosomal components: studies using novel approaches. Cell. 1976 Dec;9(4 Pt 2):633–644. doi: 10.1016/0092-8674(76)90127-6. [DOI] [PubMed] [Google Scholar]
- Smith I., Dubnau D., Morrell P., Marmur J. Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis. J Mol Biol. 1968 Apr 14;33(1):123–140. doi: 10.1016/0022-2836(68)90285-4. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- de Wachter R., Fiers W. Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA. Anal Biochem. 1972 Sep;49(1):184–197. doi: 10.1016/0003-2697(72)90257-6. [DOI] [PubMed] [Google Scholar]