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. 1979 Dec;140(3):859–863. doi: 10.1128/jb.140.3.859-863.1979

Presence and synthesis of cholesterol in stable staphylococcal L-forms.

M Hayami, A Okabe, K Sasai, H Hayashi, Y Kanemasa
PMCID: PMC216726  PMID: 533769

Abstract

The sterol which was present in two strains of a stable staphylococcal L-form was analyzed by gas-liquid chromatography and combined gas-liquid chromatography-mass spectrometry. The retention time of the sterol on gas-liquid chromatography was the same as that of authentic cholesterol. Analysis of the sterol by mass spectrometry showed a molecular ion at an m/e of 386 and the same patterns of major ions above an m/e of 145 as those of authentic cholesterol. As a result, the sterol in staphylococcal L-form was identified as cholesterol. A parent strain and its L-forms were cultured in medium containing [14C]acetate, and the synthesis of cholesterol was examined. In the L-forms, 0.52% of the total lipid radioactivity was found in cholesterol fraction, whereas no significant radioactivity was detected in the cholesterol fraction of the parent strain, indicating that staphylococcal L-forms have acquired the capacity to synthesize cholesterol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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