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. 1979 Dec;140(3):1008–1012. doi: 10.1128/jb.140.3.1008-1012.1979

Effect of intracellular carbohydrates on heat resistance of Dictyostelium discoideum spores.

R G Emyanitoff, B E Wright
PMCID: PMC216745  PMID: 533762

Abstract

The effect of intracellular trehalose and glycogen on the survival of spores of Dictyostelium discoideum ATCC 25697 after exposure to supraoptimal temperatures was examined. Cells metabolically perturbed by incubation in glucose and inorganic phosphate have intracellular trehalose and glycogen concentrations fivefold and twofold higher, respectively, than those of the controls. These cells were more resistant to the lethal effects of wet heat (45 degrees to 55 degrees C) than were control cells. The presence of 40 mM trehalose in the buffer during heat stress increased the survival of nonperturbed cells to approximately the level of the perturbed cells. No protection was observed when cells were heated in the presence of exogenous glycogen. Glucose or disaccharides other than trehalose when present during heat stress, had no effect on heat resistance. Nonperturbed cells preincubated in 40 mM trehalose and washed before heat stress were more resistant to killing than were controls. Cells perturbed with inorganic phosphate, which has been shown to increase trehalose concentrations but decrease glycogen concentrations, were also more resistant to the lethal effects of wet heat than were controls. The data suggest that trehalose has an effect on the wet-heat resistance of D. discoideum. Some possible mechanisms are suggested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Budd K., Sussman A. S., Eilers F. I. Glucose-C14 metabolism of dormant and activated ascospores of Neurospora. J Bacteriol. 1966 Feb;91(2):551–561. doi: 10.1128/jb.91.2.551-561.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ceccarini C., Filosa M. Carbohydrate content during development of the slime mold, Dictyostelium discoideum. J Cell Physiol. 1965 Oct;66(2):135–140. doi: 10.1002/jcp.1030660202. [DOI] [PubMed] [Google Scholar]
  3. Cotter D. A., Raper K. B. Spore germination in Dictyostelium discoideum: trehalase and the requirement for protein synthesis. Dev Biol. 1970 May;22(1):112–128. doi: 10.1016/0012-1606(70)90009-6. [DOI] [PubMed] [Google Scholar]
  4. Gould G. W., Dring G. J. Heat resistance of bacterial endospores and concept of an expanded osmoregulatory cortex. Nature. 1975 Dec 4;258(5534):402–405. doi: 10.1038/258402a0. [DOI] [PubMed] [Google Scholar]
  5. Horikoshi K., Ikeda Y. Trehalase in conidia of Aspergillus oryzae. J Bacteriol. 1966 May;91(5):1883–1887. doi: 10.1128/jb.91.5.1883-1887.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Killick K. A., Wright B. E. Trehalose synthesis during differentiation in Dictyostelium discoideum. Preparation, stabilization and assay of trehalose-6-phosphate synthetase. Arch Biochem Biophys. 1975 Oct;170(2):634–643. doi: 10.1016/0003-9861(75)90159-9. [DOI] [PubMed] [Google Scholar]
  7. LIDDEL G. U., WRIGHT B. E. The effect of glucose on respiration of the differentiating slime mold. Dev Biol. 1961 Jun;3:265–276. doi: 10.1016/0012-1606(61)90047-1. [DOI] [PubMed] [Google Scholar]
  8. Müller U., Hohl H. R. Ultrastructural evidence for the presence of two separate glycogen pools in Dictyostelium discoideum. Protoplasma. 1975;85(2-4):199–207. doi: 10.1007/BF01567946. [DOI] [PubMed] [Google Scholar]
  9. Rosness P. A., Wright B. E. In vivo changes of cellulose, trehalose and glycogen during differentiation of Dictyostelium discoideum. Arch Biochem Biophys. 1974 Sep;164(1):60–72. doi: 10.1016/0003-9861(74)90008-3. [DOI] [PubMed] [Google Scholar]
  10. Rousseau P., Halvorson H. O. Physiological changes following the breaking of dormancy of Saccharomyces cerevisiae ascospores. Can J Microbiol. 1973 May;19(5):547–555. doi: 10.1139/m73-091. [DOI] [PubMed] [Google Scholar]
  11. SUSSMAN A. S. The role of trehalose in the activation of dormant ascospores of neurospora. Q Rev Biol. 1961 Jun;36:109–116. doi: 10.1086/403332. [DOI] [PubMed] [Google Scholar]
  12. Sussman A. S. The dormancy and germination of fungus spores. Symp Soc Exp Biol. 1969;23:99–121. [PubMed] [Google Scholar]
  13. WHITE G. J., SUSSMAN M. Polysaccharides involved in slimemold development. I. Water-soluble glucose polymer (s). Biochim Biophys Acta. 1963 Jul 16;74:173–178. doi: 10.1016/0006-3002(63)91355-6. [DOI] [PubMed] [Google Scholar]
  14. Wright B. E., Killick K. A., Tai A. Fourth expansion and glucose perturbation of the Dictyostelium kinetic model. Eur J Biochem. 1977 Apr 1;74(2):217–225. doi: 10.1111/j.1432-1033.1977.tb11384.x. [DOI] [PubMed] [Google Scholar]
  15. Wright B. E., Tai A., Killick K. A., Thomas D. A. The effects of exogenous glucose, uracil, and inorganic phosphate on differentiation in Dictyostelium discoideum. Arch Biochem Biophys. 1979 Feb;192(2):489–499. doi: 10.1016/0003-9861(79)90119-x. [DOI] [PubMed] [Google Scholar]

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