Abstract
Candida utilis has been shown to contain 4.7 pmol of cobalamin per g of wet cell paste. Purification of the cobalamin showed it to be a mixture of methylcobalamin and adenosylcobalamin. Two cobalamin-dependent enzyme systems have been found in the yeast: methylcobalamin-dependent methionine biosynthesis and leucine 2,3-aminomutase. The cobalamin extracted from the yeast is as effective as authentic adenosylcobalamin in stimulating leucine 2,3-aminomutase.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARKER H. A., SMYTH R. D., WEISSBACH H., MUNCH-PETERSEN A., TOOHEY J. I., LADD J. N., VOLCANI B. E., WILSON R. M. Assay, purification, and properties of the adenylocobamide coenzyme. J Biol Chem. 1960 Jan;235:181–190. [PubMed] [Google Scholar]
- FRIEDEN C. Glutamate dehydrogenase. IV. Studies on enzyme inactivation and coenzyme binding. J Biol Chem. 1963 Jan;238:146–154. [PubMed] [Google Scholar]
- Fenton W. A., Rosenberg L. E. Improved techniques for the extraction and chromatography of cobalamins. Anal Biochem. 1978 Oct 1;90(1):119–125. doi: 10.1016/0003-2697(78)90014-3. [DOI] [PubMed] [Google Scholar]
- Gehrke C. W., Leimer K. Trimethylsilylation of amino acids derivatization and chromatography. J Chromatogr. 1971 May 6;57(2):219–238. doi: 10.1016/0021-9673(71)80035-3. [DOI] [PubMed] [Google Scholar]
- HALBROOK E. R., CORDS F., WINTER A. R., SUTTON T. S. Vitamin B12 production by microorganisms isolated from poultry house litter and droppings. J Nutr. 1950 Aug 10;41(4):555–563. doi: 10.1093/jn/41.4.555. [DOI] [PubMed] [Google Scholar]
- Heyssel R. M., Bozian R. C., Darby W. J., Bell M. C. Vitamin B12 turnover in man. The assimilation of vitamin B12 from natural foodstuff by man and estimates of minimal daily dietary requirements. Am J Clin Nutr. 1966 Mar;18(3):176–184. doi: 10.1093/ajcn/18.3.176. [DOI] [PubMed] [Google Scholar]
- Kolhouse J. F., Kondo H., Allen N. C., Podell E., Allen R. H. Cobalamin analogues are present in human plasma and can mask cobalamin deficiency because current radioisotope dilution assays are not specific for true cobalamin. N Engl J Med. 1978 Oct 12;299(15):785–792. doi: 10.1056/NEJM197810122991501. [DOI] [PubMed] [Google Scholar]
- Linnell J. C., Hussein H. A., Matthews D. M. A two-dimensional chromato-bioautographic method for complete separation of individual plasma cobalamins. J Clin Pathol. 1970 Dec;23(9):820–821. doi: 10.1136/jcp.23.9.820. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Linnell J. C., Mackenzie H. M., Wilson J., Matthews D. M. Patterns of plasma cobalamins in control subjects and in cases of vitamin B12 deficiency. J Clin Pathol. 1969 Sep;22(5):545–550. doi: 10.1136/jcp.22.5.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nexo E. Characterization of the cobalamins attached to transcobalamin I and transcobalamin II in human plasma. Scand J Haematol. 1977 May;18(5):358–360. [PubMed] [Google Scholar]
- Nexø E., Andersen J. Unsaturated and cobalamin saturated transcobalamin I and II in normal human plasma. Scand J Clin Lab Invest. 1977 Dec;37(8):723–728. doi: 10.3109/00365517709101856. [DOI] [PubMed] [Google Scholar]
- Poston J. M. Leucine 2,3-aminomutase, an enzyme of leucine catabolism. J Biol Chem. 1976 Apr 10;251(7):1859–1863. [PubMed] [Google Scholar]
- Poston J. M. Leucine 2,3-aminomutase: a cobalamin-dependent enzyme present in bean seedlings. Science. 1977 Jan 21;195(4275):301–302. doi: 10.1126/science.195.4275.301. [DOI] [PubMed] [Google Scholar]
- SCHEID H. E., ANDREWS M. M., SCHWEIGERT B. S. Comparison of methods for determination of the vitamin B12 potency of meats. J Nutr. 1952 Aug 11;47(4):601–610. doi: 10.1093/jn/47.4.601. [DOI] [PubMed] [Google Scholar]
- WEISSBACH H., REDFIELD B. G., DICKERMAN H. EFFECT OF VITAMIN B12 ANALOGUES ON METHIONINE FORMATION FROM N5-METHYLTETRAHYDROFOLIC ACID. J Biol Chem. 1964 Jan;239:146–148. [PubMed] [Google Scholar]