Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Oct;140(1):37–42. doi: 10.1128/jb.140.1.37-42.1979

Transformation and transfection of Pseudomonas aeruginosa: effects of metal ions.

A A Mercer, J S Loutit
PMCID: PMC216776  PMID: 115840

Abstract

The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 promoted transfection of P. aeruginosa by the linear deoxyribonucleic acid of phage F116. CaCl2 was demonstrated to allow adsorption and entry into the cell of F116 deoxyribonucleic acid such that it became resistant to exogenous deoxyribonuclease, but phage production occurred only when MgCl2 was provided. Inactivation of linear phage deoxyribonucleic acid taken up in the absence of MgCl2 was observed. The transfection frequencies at various concentrations of MgCl2 were compared, and the optimum response occurred at the concentration which promoted the highest frequency of transformation by RP1 deoxyribonucleic acid.

Full text

PDF
37

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benzinger R. Transfection of Enterobacteriaceae and its applications. Microbiol Rev. 1978 Mar;42(1):194–236. doi: 10.1128/mr.42.1.194-236.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Booker R. J., Loutit J. S. The order of replication of chromosomal markers in Pseudomonas aeruginosa strain 1. I. Marker frequency analysis by transduction. Genet Res. 1974 Apr;23(2):145–153. doi: 10.1017/s0016672300014762. [DOI] [PubMed] [Google Scholar]
  3. Chakrabarty A. M., Mylroie J. R., Friello D. A., Vacca J. G. Transformation of Pseudomonas putida and Escherichia coli with plasmid-linked drug-resistance factor DNA. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3647–3651. doi: 10.1073/pnas.72.9.3647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cosloy S. D., Oishi M. The nature of the transformation process in Escherichia coli K12. Mol Gen Genet. 1973 Jul 31;124(1):1–10. doi: 10.1007/BF00267159. [DOI] [PubMed] [Google Scholar]
  6. HOLLOWAY B. W., EGAN J. B., MONK M. Lysogeny in Pseudomonas aeruginosa. Aust J Exp Biol Med Sci. 1960 Aug;38:321–329. doi: 10.1038/icb.1960.34. [DOI] [PubMed] [Google Scholar]
  7. Khan N. C., Sen S. P. Genetic transformation in Pseudomonas. J Gen Microbiol. 1967 Nov;49(2):201–209. doi: 10.1099/00221287-49-2-201. [DOI] [PubMed] [Google Scholar]
  8. Lederberg E. M., Cohen S. N. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol. 1974 Sep;119(3):1072–1074. doi: 10.1128/jb.119.3.1072-1074.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Loutit J. S., Marinus M. G. Investigation of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genet Res. 1968 Aug;12(1):37–44. doi: 10.1017/s0016672300011599. [DOI] [PubMed] [Google Scholar]
  10. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  11. Marinus M. G., Loutit J. S. Regulation of isoleucine-valine biosynthesis in Pseudomonas aeruginosa. I. Characterisation and mapping of mutants. Genetics. 1969 Nov;63(3):547–556. doi: 10.1093/genetics/63.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Miller R. V., Clark A. J. Purification and properties of two deoxyribonucleases of Pseudomonas aeruginosa. J Bacteriol. 1976 Aug;127(2):794–802. doi: 10.1128/jb.127.2.794-802.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller R. V., Pemberton J. M., Richards K. E. F116, D3 and G101: temperate bacteriophages of Pseudomonas aeruginosa. Virology. 1974 Jun;59(2):566–569. doi: 10.1016/0042-6822(74)90466-8. [DOI] [PubMed] [Google Scholar]
  15. Pemberton J. M., Clark A. J. Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO. J Bacteriol. 1973 Apr;114(1):424–433. doi: 10.1128/jb.114.1.424-433.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sjöström J. E., Lindberg M., Philipson L. Transfection of Staphylococcus aureus with bacteriophage deoxyribonucleic acid. J Bacteriol. 1972 Jan;109(1):285–291. doi: 10.1128/jb.109.1.285-291.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stanisich V. A., Bennett P. M. The properties of hybrids formed between the P-group plasmid RP1 and various plasmids from Pseudomonas aeruginosa. Mol Gen Genet. 1976 Dec 8;149(2):217–223. doi: 10.1007/BF00332892. [DOI] [PubMed] [Google Scholar]
  18. Taketo A. Sensitivity of Escherichia coli to viral nucleic acid. 8. Idiosyncrasy of Ca2+-dependent competence for DNA. J Biochem. 1974 Apr;75(4):895–904. doi: 10.1093/oxfordjournals.jbchem.a130463. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES