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. 1979 Oct;140(1):43–49. doi: 10.1128/jb.140.1.43-49.1979

Deoxyribonucleic acid and outer membrane: binding to outer membrane involves a specific protein.

H Wolf-Watz, A Norqvist
PMCID: PMC216777  PMID: 227834

Abstract

The binding of deoxyribonucleic acid (DNA) to the outer membrane of Escherichia coli was examined. The amount of DNA found to be bound to outer membrane was low and was estimated to be about 0.4% of the total DNA. Treatment of cells with chloramphenicol or rifampin caused a disassociation of the apparent DNA-outer membrane complex. The results presented here suggest that the binding between membrane and DNA is specific and involves a membrane protein having a molecular weight of 13,000.

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Selected References

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  1. Abe M., Brown C., Hendrickson W. G., Boyd D. H., Clifford P., Cote R. H., Schaechter M. Release of Escherichia coli DNA from membrane complexes by single-strand endonucleases. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2756–2760. doi: 10.1073/pnas.74.7.2756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailey S. C., Apirion D. Identification of lipopolysaccharides and phospholipids of Escherichia coli in polyacrylamide gels. J Bacteriol. 1977 Jul;131(1):347–355. doi: 10.1128/jb.131.1.347-355.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bayer M. E. Adsorption of bacteriophages to adhesions between wall and membrane of Escherichia coli. J Virol. 1968 Apr;2(4):346–356. doi: 10.1128/jvi.2.4.346-356.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cozzarelli N. R. The mechanism of action of inhibitors of DNA synthesis. Annu Rev Biochem. 1977;46:641–668. doi: 10.1146/annurev.bi.46.070177.003233. [DOI] [PubMed] [Google Scholar]
  5. Craine B. L., Rupert C. S. Deoxyribonucleic acid-membrane interactions near the origin of replication and initiation of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1979 Feb;137(2):740–745. doi: 10.1128/jb.137.2.740-745.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Craine B. L., Rupert C. S. Identification of a biochemically unique DNA-membrane interaction involving the Escherichia coli origin of replication. J Bacteriol. 1978 Apr;134(1):193–199. doi: 10.1128/jb.134.1.193-199.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dworsky P. Einfluss von Inhibitoren der Protein- und Nucleinsäuresynthese auf die Gestalt des Nucleoids von Escherichia coli. Z Allg Mikrobiol. 1974;14(1):3–28. doi: 10.1002/jobm.3630140102. [DOI] [PubMed] [Google Scholar]
  8. Dworsky P., Schaechter M. Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli. J Bacteriol. 1973 Dec;116(3):1364–1374. doi: 10.1128/jb.116.3.1364-1374.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  10. Fielding P., Fox C. F. Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli. Biochem Biophys Res Commun. 1970 Oct 9;41(1):157–162. doi: 10.1016/0006-291x(70)90482-1. [DOI] [PubMed] [Google Scholar]
  11. Gomez-Eichelmann M. C., Bastarrachea F. Progress in the resolution of the cytoplasmic membrane DNA initiation complex of Escherichia coli. Biochim Biophys Acta. 1975 Oct 15;407(3):273–282. doi: 10.1016/0005-2787(75)90094-5. [DOI] [PubMed] [Google Scholar]
  12. Heidrich H. G., Olsen W. L. Deoxyribonucleic acid-envelope complexes from Escherichia coli. A complex-specific protein and its possible function for the stability of the complex. J Cell Biol. 1975 Nov;67(2PT1):444–460. doi: 10.1083/jcb.67.2.444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  14. Morgan C., Rosenkranz H. S., Carr H. S., Rose H. M. Electron microscopy of chloramphenicol-treated Escherichia coli. J Bacteriol. 1967 Jun;93(6):1987–2002. doi: 10.1128/jb.93.6.1987-2002.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nicolaidis A. A., Holland I. B. Evidence for the specific association of the chromosomal origin with outer membrane fractions isolated from Escherichia coli. J Bacteriol. 1978 Jul;135(1):178–189. doi: 10.1128/jb.135.1.178-189.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Olsen W. L., Heidrich H. G., Hannig K., Hofschneider P. H. Deoxyribonucleic acid-envelope complexes isolated from Escherichia coli by free-flow electrophoresis: biochemical and electron microscope characterization. J Bacteriol. 1974 May;118(2):646–653. doi: 10.1128/jb.118.2.646-653.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  18. Parker D. L., Glaser D. A. Chromosomal sites of DNA-membrane attachment in Escherichia coli. J Mol Biol. 1974 Aug 5;87(2):153–168. doi: 10.1016/0022-2836(74)90140-5. [DOI] [PubMed] [Google Scholar]
  19. Parker D. L., Glaser D. A. Effect of growth conditions on DNA-membrane attachment in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2446–2450. doi: 10.1073/pnas.72.6.2446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pato M. L. Alterations of the rate of movement of deoxyribonucleic acid replication forks. J Bacteriol. 1975 Jul;123(1):272–277. doi: 10.1128/jb.123.1.272-277.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Portalier R., Worcel A. Association of the folded chromosome with the cell envelope of E. coli: characterization of the proteins at the DNA-membrane attachment site. Cell. 1976 Jun;8(2):245–255. doi: 10.1016/0092-8674(76)90008-8. [DOI] [PubMed] [Google Scholar]
  22. Stonington O. G., Pettijohn D. E. The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex. Proc Natl Acad Sci U S A. 1971 Jan;68(1):6–9. doi: 10.1073/pnas.68.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  24. Wolf-Watz H., Masters M. Deoxyribonucleic acid and outer membrane: strains diploid for the oriC region show elevated levels of deoxyribonucleic acid-binding protein and evidence for specific binding of the oriC region to outer membrane. J Bacteriol. 1979 Oct;140(1):50–58. doi: 10.1128/jb.140.1.50-58.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Zusman D. R., Carbonell A., Haga J. Y. Nucleoid condensation and cell division in Escherichia coli MX74T2 ts52 after inhibition of protein synthesis. J Bacteriol. 1973 Sep;115(3):1167–1178. doi: 10.1128/jb.115.3.1167-1178.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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