Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Oct;140(1):65–72. doi: 10.1128/jb.140.1.65-72.1979

Macromolecular synthesis in synchronized cultures of Anacystis nidulans.

Y Asato
PMCID: PMC216780  PMID: 115841

Abstract

Synchronous culture of Anacystis nidulans has been induced by the light-dark-light regimen. At various time intervals during synchronous growth, samples were pulsed with radioactive labels to determine phospholipid, protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA) syntheses within the cell division cycle. A temporal order of protein, RNA, and DNA syntheses occurred within the cell division cycle, whereas phospholipid was characteristically synthesized during midcycle (during cell enlargement) and during the time of cell division. Chemically determined protein, RNA, and DNA syntheses were found to support the schedule of these macromolecules in cultures growing at an 8-h doubling time.

Full text

PDF
65

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen M. M. Ultrastructure of the cell wall and cell division of unicellular blue-green algae. J Bacteriol. 1968 Sep;96(3):842–852. doi: 10.1128/jb.96.3.842-852.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asato Y., Folsome C. E. Temporal genetic mapping of the blue-green alga, Anacystis nidulans. Genetics. 1970 Jul;65(3):407–419. doi: 10.1093/genetics/65.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CERIOTTI G. Determination of nucleic acids in animal tissues. J Biol Chem. 1955 May;214(1):59–70. [PubMed] [Google Scholar]
  4. Chai N. C., Lark K. G. Cytological studies of deoxyribonucleic acid replication in Escherichia coli 15T-: replication at slow growth rates and after a shift-up into rich medium. J Bacteriol. 1970 Oct;104(1):401–409. doi: 10.1128/jb.104.1.401-409.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  6. Csatorday K., Horváth G. Synchronization of Anacystis nidulans. Oxygen evolution during the cell cycle. Arch Microbiol. 1977 Jan 11;111(3):245–246. doi: 10.1007/BF00549361. [DOI] [PubMed] [Google Scholar]
  7. Delaney S. F., Carr N. G. Temporal genetic mapping in the blue-green alga Anacystis nidulans using ethyl methanesulphonate. J Gen Microbiol. 1975 Jun;88(2):259–268. doi: 10.1099/00221287-88-2-259. [DOI] [PubMed] [Google Scholar]
  8. Helmstetter C. E., Pierucci O. DNA synthesis during the division cycle of three substrains of Escherichia coli B/r. J Mol Biol. 1976 Apr 15;102(3):477–486. doi: 10.1016/0022-2836(76)90329-6. [DOI] [PubMed] [Google Scholar]
  9. Herdman M., Carr N. G. Observations on replication and cell division in synchronous cultures of the blue-green alga Anacystis nidulans. J Bacteriol. 1971 Aug;107(2):583–584. doi: 10.1128/jb.107.2.583-584.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KECK K. An ultramicro technique for the determination of deoxypentose nucleic acid. Arch Biochem Biophys. 1956 Aug;63(2):446–451. doi: 10.1016/0003-9861(56)90059-5. [DOI] [PubMed] [Google Scholar]
  11. Kubitschek H. E., Bendigkeit H. E., Loken M. R. Onset of DNA synthesis during the cell cycle in chemostat cultures. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1611–1617. doi: 10.1073/pnas.57.6.1611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kubitschek H. E., Freedman M. L. Chromosome replication and the division cycle of Escherichia coli B-r. J Bacteriol. 1971 Jul;107(1):95–99. doi: 10.1128/jb.107.1.95-99.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kubitschek H. E., Newman C. N. Chromosome replication during the division cycle in slowly growing, steady-state cultures of three Escherichia coli B/r strains. J Bacteriol. 1978 Oct;136(1):179–190. doi: 10.1128/jb.136.1.179-190.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Lark C. Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates. Biochim Biophys Acta. 1966 Jun 22;119(3):517–525. doi: 10.1016/0005-2787(66)90128-6. [DOI] [PubMed] [Google Scholar]
  16. Mann N., Carr N. G. Coupling between the initiation of DNA replication and cell division in the blue-green alga Anacystis nidulans. Arch Microbiol. 1977 Feb 4;112(1):95–98. doi: 10.1007/BF00446659. [DOI] [PubMed] [Google Scholar]
  17. Sueoka N., Chiang K. S., Kates J. R. Deoxyribonucleic acid replication in meiosis of Chlamydomonas reinhardi. I. Isotopic transfer experiments with a strain producing eight zoospores. J Mol Biol. 1967 Apr 14;25(1):47–66. doi: 10.1016/0022-2836(67)90278-1. [DOI] [PubMed] [Google Scholar]
  18. Venkatarman G. S., Lorenzen H. Biochemical studies on Anacystis nidulans during its synchronous growth. Arch Mikrobiol. 1969;69(1):34–39. doi: 10.1007/BF00408560. [DOI] [PubMed] [Google Scholar]
  19. Zusman D. R., Krotoski D. M., Cumsky M. Chromosome replication in Myxococcus xanthus. J Bacteriol. 1978 Jan;133(1):122–129. doi: 10.1128/jb.133.1.122-129.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zusman D., Rosenberg E. DNA cycle of Myxococcus xanthus. J Mol Biol. 1970 May 14;49(3):609–619. doi: 10.1016/0022-2836(70)90285-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES