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. 1979 Oct;140(1):188–196. doi: 10.1128/jb.140.1.188-196.1979

Products of endogenous N-acetylglucosaminyltransferase activity of Dictyostelium discoideum during growth and early development.

E V Crean, J P Lagerstedt, E F Rossomando
PMCID: PMC216795  PMID: 500556

Abstract

In the presence of Mn2+ and uridine diphosphate-N-acetyl-D-[14C]glucosamine, a total particulate fraction prepared from Dictyostelium discoideum amoebae catalyzed the transfer of N-acetyl[14C]glucosamine to endogenous membrane protein acceptors. No transfer to lipid acceptors was evident. The 14C products obtained from growth-phase and aggregation-competent amoebae were converted to glycopeptides by pronase digestion. The respective glycopeptides appeared identical in their chemical and chromatographic properties, suggesting that the same activity was functioning in both growing and differentiating cells. The results provided no evidence for developmental regulation of this activity in D. discoideum.

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Selected References

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