Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Oct;140(1):197–205. doi: 10.1128/jb.140.1.197-205.1979

Membrane H+ conductance of Streptococcus lactis.

P C Maloney
PMCID: PMC216796  PMID: 40951

Abstract

Membrane conductance to H+ was measured in the anaerobic bacterium Streptoccus lactis by a pulse technique employing a low driving force (0.1 pH unit; 6 mV). Over the pH range of 3.7 to 8.5, a constant value for passive H+ conductance was observed, corresponding to 0.2 mumol of H+/s per p/ unit per g, dry weight (1.6 microS/cm2 of surface area). The pH insensitivity of this low basal H+ conductance supports the idea that a circulation of protons can mediate highly efficiency engery transductions across the membranes of bacteria.

Full text

PDF
197

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrams A. The release of bound adenosine triphosphatase from isolated bacterial membranes and the properties of the solubilized enzyme. J Biol Chem. 1965 Sep;240(9):3675–3681. [PubMed] [Google Scholar]
  2. Altendorf K., Harold F. M., Simoni R. D. Impairment and restoration of the energized state in membrane vesicles of a mutant of Escherichia coli lacking adenosine triphosphatase. J Biol Chem. 1974 Jul 25;249(14):4587–4593. [PubMed] [Google Scholar]
  3. Bakker E. P. Accumulation of thallous ions (Tl+) as a measure of the electrical potential difference across the cytoplasmic membrane of bacteria. Biochemistry. 1978 Jul 11;17(14):2899–2904. doi: 10.1021/bi00607a031. [DOI] [PubMed] [Google Scholar]
  4. Bashford C. L., Thayer W. S. Thermodynamics of the electrochemical proton gradient in bovine heart submitochondrial particles. J Biol Chem. 1977 Dec 10;252(23):8459–8463. [PubMed] [Google Scholar]
  5. Collins S. H., Hamilton W. A. Magnitude of the protonmotive force in respiring Staphylococcus aureus and Escherichia coli. J Bacteriol. 1976 Jun;126(3):1224–1231. doi: 10.1128/jb.126.3.1224-1231.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fillingame R. H. Identification of the dicyclohexylcarbodiimide-reactive protein component of the adenosine 5'-triphosphate energy-transducing system of Escherichia coli. J Bacteriol. 1975 Nov;124(2):870–883. doi: 10.1128/jb.124.2.870-883.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GILBY A. R., FEW A. V. The permeability of Micrococcus lysodeikticus to hydrochloric acid. Biochim Biophys Acta. 1958 Nov;30(2):421–422. doi: 10.1016/0006-3002(58)90066-0. [DOI] [PubMed] [Google Scholar]
  8. Harold F. M., Baarda J. R., Baron C., Abrams A. Inhibition of membrane-bound adenosine triphosphatase and of cation transport in Streptococcus faecalis by N,N'-dicyclohexylcarbodiimide. J Biol Chem. 1969 May 10;244(9):2261–2268. [PubMed] [Google Scholar]
  9. Harold F. M. Ion currents and physiological functions in microorganisms. Annu Rev Microbiol. 1977;31:181–203. doi: 10.1146/annurev.mi.31.100177.001145. [DOI] [PubMed] [Google Scholar]
  10. Harold F. M., Papineau D. Cation transport and electrogenesis by Streptococcus faecalis. I. The membrane potential. J Membr Biol. 1972;8(1):27–44. doi: 10.1007/BF01868093. [DOI] [PubMed] [Google Scholar]
  11. Henn F. A., Thompson T. E. Synthetic lipid bilayer membranes. Annu Rev Biochem. 1969;38:241–262. doi: 10.1146/annurev.bi.38.070169.001325. [DOI] [PubMed] [Google Scholar]
  12. Hinkle P. C., Horstman L. L. Respiration-driven proton transport in submitochondrial particles. J Biol Chem. 1971 Oct 10;246(19):6024–6028. [PubMed] [Google Scholar]
  13. Hirata H., Altendorf K., Harold F. M. Role of an electrical potential in the coupling of metabolic energy to active transport by membrane vesicles of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1804–1808. doi: 10.1073/pnas.70.6.1804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hopfer U., Lehninger A. L., Thompson T. E. Protonic conductance across phospholipid bilayer membranes induced by uncoupling agents for oxidative phosphorylation. Proc Natl Acad Sci U S A. 1968 Feb;59(2):484–490. doi: 10.1073/pnas.59.2.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kashket E. R., Wilson T. H. Role of metabolic energy in the transport of -galactosides by Streptococcus lactis. J Bacteriol. 1972 Feb;109(2):784–789. doi: 10.1128/jb.109.2.784-789.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Maloney P. C. Coupling between H+ entry and ATP formation in Escherichia coli. Biochem Biophys Res Commun. 1978 Aug 29;83(4):1496–1501. doi: 10.1016/0006-291x(78)91390-6. [DOI] [PubMed] [Google Scholar]
  17. Maloney P. C., Kashket E. R., Wilson T. H. A protonmotive force drives ATP synthesis in bacteria. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3896–3900. doi: 10.1073/pnas.71.10.3896. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Maloney P. C. Obligatory coupling between proton entry and the synthesis of adenosine 5'-triphosphate in Streptococcus lactis. J Bacteriol. 1977 Nov;132(2):564–575. doi: 10.1128/jb.132.2.564-575.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Maloney P. C., Wilson T. H. ATP synthesis driven by a protonmotive force in Streptococcus lactis. J Membr Biol. 1975;25(3-4):285–310. doi: 10.1007/BF01868580. [DOI] [PubMed] [Google Scholar]
  20. Mitchell P., Moyle J. Acid-base titration across the membrane system of rat-liver mitochondria. Catalysis by uncouplers. Biochem J. 1967 Aug;104(2):588–600. doi: 10.1042/bj1040588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mitchell P., Moyle J. Estimation of membrane potential and pH difference across the cristae membrane of rat liver mitochondria. Eur J Biochem. 1969 Feb;7(4):471–484. doi: 10.1111/j.1432-1033.1969.tb19633.x. [DOI] [PubMed] [Google Scholar]
  22. Mitchell P., Moyle J. Respiration-driven proton translocation in rat liver mitochondria. Biochem J. 1967 Dec;105(3):1147–1162. doi: 10.1042/bj1051147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mitchell P. Vectorial chemistry and the molecular mechanics of chemiosmotic coupling: power transmission by proticity. Biochem Soc Trans. 1976;4(3):399–430. doi: 10.1042/bst0040399. [DOI] [PubMed] [Google Scholar]
  24. Nicholls D. G. The influence of respiration and ATP hydrolysis on the proton-electrochemical gradient across the inner membrane of rat-liver mitochondria as determined by ion distribution. Eur J Biochem. 1974 Dec 16;50(1):305–315. doi: 10.1111/j.1432-1033.1974.tb03899.x. [DOI] [PubMed] [Google Scholar]
  25. Okamoto H., Sone N., Hirata H., Yoshida M., Kagawa Y. Purified proton conductor in proton translocating adenosine triphosphatase of a thermophilic bacterium. J Biol Chem. 1977 Sep 10;252(17):6125–6131. [PubMed] [Google Scholar]
  26. Patel L., Kaback H. R. The role of the carbodiimide-reactive component of the adenosine-5'-triphosphatase complex in the proton permeability of Escherichia coli membrane vesicles. Biochemistry. 1976 Jun 29;15(13):2741–2746. doi: 10.1021/bi00658a005. [DOI] [PubMed] [Google Scholar]
  27. Ramos S., Schuldiner S., Kaback H. R. The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1892–1896. doi: 10.1073/pnas.73.6.1892. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Roisin M. P., Kepes A. The membrane ATPase of Escherichia coli. I. Release into solution, allotopic properties and reconstitution of membrane-bound ATPase. Biochim Biophys Acta. 1973 May 30;305(2):249–259. doi: 10.1016/0005-2728(73)90173-4. [DOI] [PubMed] [Google Scholar]
  29. Rosen B. P. Beta-galactoside transport and proton movements in an adenosine triphosphatase-deficient mutant of Escherichia coli. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1289–1296. doi: 10.1016/0006-291x(73)90605-0. [DOI] [PubMed] [Google Scholar]
  30. Scholes P., Mitchell P. Acid-base titration across the plasma membrane of Micrococcus denitrificans: factors affecting the effective proton conductance and the respiratory rate. J Bioenerg. 1970 Jun;1(1):61–72. doi: 10.1007/BF01516089. [DOI] [PubMed] [Google Scholar]
  31. Thayer W. S., Tu Y. S., Hinkle P. C. Thermodynamics of oxidative phosphorylation in bovine heart submitochondrial particles. J Biol Chem. 1977 Dec 10;252(23):8455–8458. [PubMed] [Google Scholar]
  32. Zilberstein D., Schuldiner S., Padan E. Proton electrochemical gradient in Escherichia coli cells and its relation to active transport of lactose. Biochemistry. 1979 Feb 20;18(4):669–673. doi: 10.1021/bi00571a018. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES