Abstract
Electron microscopic heteroduplex analysis and comparative restriction digests have been used to characterize lambda p123(209), the complementary pair of phages used in the Casadaban technique of gene fusion. Derivatives of lambda 1(209) constructed to carry fusions of the lac genes to the control regions of the ilvC and ilvEDA operons were also analyzed. These physical maps have provided confirmation of the genetic models for these constructions and physical specifications important in interpreting the behavior of these ilv-lac fusions.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allet B., Bukhari A. I. Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases. J Mol Biol. 1975 Mar 15;92(4):529–540. doi: 10.1016/0022-2836(75)90307-1. [DOI] [PubMed] [Google Scholar]
- Allet B. Nucleotide sequences at the ends of bacteriophage Mu DNA. Nature. 1978 Aug 10;274(5671):553–558. doi: 10.1038/274553a0. [DOI] [PubMed] [Google Scholar]
- Avitabile A., Carlomagno-Cerillo S., Favvre R., Blasi F. Isolation of transducing bacteriophages for the histidine and isoleucine-valine operons in Escherichia coli K-12. J Bacteriol. 1972 Oct;112(1):40–47. doi: 10.1128/jb.112.1.40-47.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
- Childs G. J., Ohtsubo H., Ohtsubo E., Sonnenberg F., Freundlich M. Restriction endonuclease mapping of the Escherichia coli K12 chromosome in the vicinity of the ilv genes. J Mol Biol. 1977 Nov 25;117(1):175–193. doi: 10.1016/0022-2836(77)90030-4. [DOI] [PubMed] [Google Scholar]
- Cohen B. M., Jones E. W. New Map Location of ilvO in ESCHERICHIA COLI. Genetics. 1976 Jun;83(2):201–225. doi: 10.1093/genetics/83.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCorkle G. M., Leathers T. D., Umbarger H. E. Physical organization of the ilvEDAC genes of Escherichia coli strain K-12. Proc Natl Acad Sci U S A. 1978 Jan;75(1):89–93. doi: 10.1073/pnas.75.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Patin D. W., Calhoun D. H. Mapping of ilvO loci of Escherichia coli K-12 with bacteriophage lambda dilv. J Bacteriol. 1979 Mar;137(3):1234–1242. doi: 10.1128/jb.137.3.1234-1242.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schiavo F. L., Favre R., Kasai T., Cascino A., Guardiola J., Caro L., Iaccarino M. The phi80 lambdadilv phage and its use to detect messenger RNA related to isoleucine-valine metabolism. J Mol Biol. 1975 Dec 15;99(3):353–368. doi: 10.1016/s0022-2836(75)80131-8. [DOI] [PubMed] [Google Scholar]
- Smith J. M., Smolin D. E., Umbarger H. E. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12. Mol Gen Genet. 1976 Oct 18;148(2):111–124. doi: 10.1007/BF00268374. [DOI] [PubMed] [Google Scholar]