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. 1979 Oct;140(1):267–275. doi: 10.1128/jb.140.1.267-275.1979

Role of gene flaFV on flagellar hook formation in Salmonella typhimurium.

K Kutsukake, T Suzuki, S Yamaguchi, T Iino
PMCID: PMC216805  PMID: 387724

Abstract

Nine temperature-sensitive nonflagellate mutants defective in flaFV were isolated from a strain of Salmonella typhimurium. Among them three mutants were found to produce flagella with abnormally shaped (either straight or irregularly curved) hooks at the permissive temperature. Two mutations that rendered hooks straight were located in one of the eight segments of flaFV defined by deletion mapping. The mutation that rendered hooks irregularly curved was located in a different segment. An flaR mutation was introduced into the latter mutant. At the permissive temperature, the resulting double mutant produced polyhooks whose wavelength and amplitude were both exceedingly reduced. These polyhook structures were more thermolabile than those of the flaFV+ strain. Hook protein of the former strain was shown to have a slightly positive electric charge compared with that of the latter. From these results and other available information, it is inferred that flaFV is the structural gene for the hook protein in Salmonella.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
  2. Chan R. K., Botstein D., Watanabe T., Ogata Y. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate. Virology. 1972 Dec;50(3):883–898. doi: 10.1016/0042-6822(72)90442-4. [DOI] [PubMed] [Google Scholar]
  3. Collins A. L., Stocker B. A. Salmonella typhimurium mutants generally defective in chemotaxis. J Bacteriol. 1976 Dec;128(3):754–765. doi: 10.1128/jb.128.3.754-765.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. DePamphilis M. L., Adler J. Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):384–395. doi: 10.1128/jb.105.1.384-395.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dimmitt K., Simon M. I. Purification and partial characterization of Bacillus subtilis Flagellar hooks. J Bacteriol. 1971 Oct;108(1):282–286. doi: 10.1128/jb.108.1.282-286.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hotani H., Asakura S. Growth-saturation in vitro of Salmonella flagella. J Mol Biol. 1974 Jun 25;86(2):285–300. doi: 10.1016/0022-2836(74)90019-9. [DOI] [PubMed] [Google Scholar]
  8. Icho T., Iino T. Isolation and characterization of motile Escherichia coli mutants resistant to bacteriophage chi. J Bacteriol. 1978 Jun;134(3):854–860. doi: 10.1128/jb.134.3.854-860.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. doi: 10.1146/annurev.ge.11.120177.001113. [DOI] [PubMed] [Google Scholar]
  10. Iino T., Oguchi T., Kuroiwa T. Polymorphism in a flagellar-shape mutant of Salmonella typhimurium. J Gen Microbiol. 1974 Mar;81(1):37–45. doi: 10.1099/00221287-81-1-37. [DOI] [PubMed] [Google Scholar]
  11. Kagawa H., Asakura S., Iino T. Serological study of bacterial flagellar hooks. J Bacteriol. 1973 Mar;113(3):1474–1481. doi: 10.1128/jb.113.3.1474-1481.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kagawa H., Owaribe K., Asakura S., Takahashi N. Flagellar hook protein from Salmonella SJ25. J Bacteriol. 1976 Jan;125(1):68–73. doi: 10.1128/jb.125.1.68-73.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Komeda Y., Silverman M., Matsumura P., Simon M. Genes for the hook-basal body proteins of the flagellar apparatus in Escherichia coli. J Bacteriol. 1978 May;134(2):655–667. doi: 10.1128/jb.134.2.655-667.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Komeda Y., Silverman M., Simon M. Genetic analysis of Escherichia coli K-12 region I flagellar mutants. J Bacteriol. 1977 Sep;131(3):801–808. doi: 10.1128/jb.131.3.801-808.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Komeda Y., Silverman M., Simon M. Identification of the structural gene for the hook subunit protein of Escherichia coli flagella. J Bacteriol. 1978 Jan;133(1):364–371. doi: 10.1128/jb.133.1.364-371.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kondo H., Yanagida M. Structure of straight flagellar filaments from a mutant of Escherichia coli. J Mol Biol. 1975 Aug 25;96(4):641–652. doi: 10.1016/0022-2836(75)90143-6. [DOI] [PubMed] [Google Scholar]
  17. MEYNELL E. W. A phage, phi chi, which attacks motile bacteria. J Gen Microbiol. 1961 Jun;25:253–290. doi: 10.1099/00221287-25-2-253. [DOI] [PubMed] [Google Scholar]
  18. MacPhee D. G., Stocker B. A. Suppression of amber and ochre mutants in Salmonella typhimurium by a mutant F'-1-gal factor carrying an ochre suppressor gene. J Bacteriol. 1969 Oct;100(1):240–246. doi: 10.1128/jb.100.1.240-246.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Martinez R. J., Ichiki A. T., Lundh N. P., Tronick S. R. A single amino acid substitution responsible for altered flagellar morphology. J Mol Biol. 1968 Jun 28;34(3):559–564. doi: 10.1016/0022-2836(68)90180-0. [DOI] [PubMed] [Google Scholar]
  20. Matsumura P., Silverman M., Simon M. Cloning and expression of the flagellar hook gene on hybird plasmids in minicells. Nature. 1977 Feb 24;265(5596):758–760. doi: 10.1038/265758a0. [DOI] [PubMed] [Google Scholar]
  21. Patterson-Delafield J., Martinez R. J., Stocker B. A., Yamaguchi S. A new fla gene in Salmonella typhimurium--flaR--and its mutant phenotype-superhooks. Arch Mikrobiol. 1973 Mar 26;90(2):107–120. doi: 10.1007/BF00414513. [DOI] [PubMed] [Google Scholar]
  22. Silverman M. R., Simon M. I. Flagellar assembly mutants in Escherichia coli. J Bacteriol. 1972 Nov;112(2):986–993. doi: 10.1128/jb.112.2.986-993.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Silverman M., Simon M. I. Bacterial flagella. Annu Rev Microbiol. 1977;31:397–419. doi: 10.1146/annurev.mi.31.100177.002145. [DOI] [PubMed] [Google Scholar]
  24. Silverman M., Simon M. Genetic analysis of flagellar mutants in Escherichia coli. J Bacteriol. 1973 Jan;113(1):105–113. doi: 10.1128/jb.113.1.105-113.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Smith H. O., Levine M. A phage P22 gene controlling integration of prophage. Virology. 1967 Feb;31(2):207–216. doi: 10.1016/0042-6822(67)90164-x. [DOI] [PubMed] [Google Scholar]
  26. Suzuki T., Iino T., Horiguchi T., Yamaguchi S. Incomplete flagellar structures in nonflagellate mutants of Salmonella typhimurium. J Bacteriol. 1978 Feb;133(2):904–915. doi: 10.1128/jb.133.2.904-915.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vary P. S., Stocker B. A. Nonsense motility mutants in Salmonella typhimurium. Genetics. 1973 Feb;73(2):229–245. doi: 10.1093/genetics/73.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Warrick H. M., Taylor B. L., Koshland D. E., Jr Chemotactic mechanism of Salmonella typhimurium: preliminary mapping and characterization of mutants. J Bacteriol. 1977 Apr;130(1):223–231. doi: 10.1128/jb.130.1.223-231.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yamaguchi S., Iino T., Horiguchi T., Ota K. Genetic analysis of fla and mot cistrons closely linked to H1 in Salmonella abortusequi and its derivatives. J Gen Microbiol. 1972 Apr;70(1):59–75. doi: 10.1099/00221287-70-1-59. [DOI] [PubMed] [Google Scholar]
  30. Yamaguchi S., Iino T., Kuroiwa T. Possession of flagellar hooks by some non-flagellate mutants of Salmonella abortusequi. J Gen Microbiol. 1972 Apr;70(2):299–303. doi: 10.1099/00221287-70-2-299. [DOI] [PubMed] [Google Scholar]

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