Abstract
A mutant of plasmid R100-1, which conferred cellular hypersensitivity to Hg2+ because of the insertion of Tn801 (TnA) into the gene determining synthesis of mercuric reductase enzyme, allowed further mutational events to be selected which resulted in either reversion to Hg2+ resistance (characteristic plasmid R100-1) or sensitivity at a level characteristic of plasmidless strains. Restriction endonuclease EcoRI and BamHI analysis showed that reversion to resistance resulted from loss of TnA from the R100-mer:Tn801 plasmid, whereas the change from hypersensitivity to sensitivity to Hg2+ usually resulted from deletion of part or all of Tn801 plus plasmid deoxyribonucleic acid sequences corresponding to the operator-proximal end of the mer operon.
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Selected References
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- Chandler M., Allet B., Gallay E., Boy de La Tour E., Caro L. Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1. Mol Gen Genet. 1977 Jun 24;153(3):289–295. doi: 10.1007/BF00431594. [DOI] [PubMed] [Google Scholar]
- Foster T. J., Nakahara H., Weiss A. A., Silver S. Transposon A-generated mutations in the mercuric resistance genes of plasmid R100-1. J Bacteriol. 1979 Oct;140(1):167–181. doi: 10.1128/jb.140.1.167-181.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lane D., Chandler M. Mapping of the drug resistance genes carried by the r-determinant of the R100.1 plasmid. Mol Gen Genet. 1977 Nov 29;157(1):17–23. doi: 10.1007/BF00268682. [DOI] [PubMed] [Google Scholar]
- Mickel S., Ohtsubo E., Bauer W. Heteroduplex mapping of small plasmids derived from R-factor R12: in vivo recombination occurs at IS1 insertion sequences. Gene. 1977;2(3-4):193–210. doi: 10.1016/0378-1119(77)90017-8. [DOI] [PubMed] [Google Scholar]
- Miki T., Easton A. M., Rownd R. H. Mapping of the resistance genes of the R plasmid NR1. Mol Gen Genet. 1978 Jan 17;158(3):217–224. doi: 10.1007/BF00267192. [DOI] [PubMed] [Google Scholar]
- Nisen P. D., Kopecko D. J., Chou J., Cohen S. N. Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3). J Mol Biol. 1977 Dec 25;117(4):975–978. doi: 10.1016/s0022-2836(77)80008-9. [DOI] [PubMed] [Google Scholar]
- Ohtsubo E., Rosenbloom M., Schrempf H., Goebel W., Rosen J. Site specific recombination involved in the generation of small plasmids. Mol Gen Genet. 1978 Feb 16;159(2):131–141. doi: 10.1007/BF00270886. [DOI] [PubMed] [Google Scholar]
- Reif H. J., Saedler H. IS1 is involved in deletion formation in the gal region of E. coli K12. Mol Gen Genet. 1975;137(1):17–28. doi: 10.1007/BF00332538. [DOI] [PubMed] [Google Scholar]
- Tanak N., Cramer J. H., Rownd R. H. EcoRI restriction endonuclease map of the composite R plasmid NR1. J Bacteriol. 1976 Jul;127(1):619–636. doi: 10.1128/jb.127.1.619-636.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]