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. 1979 Jul;139(1):8–12. doi: 10.1128/jb.139.1.8-12.1979

Novel acriflavin resistance genes, acrC and acrD, in Escherichia coli K-12.

H Nakamura
PMCID: PMC216820  PMID: 378962

Abstract

Acriflavine-resistant mutants were isolated from an acriflavine-sensitive (acrA) strain of Escherichia coli K-12 and then tested for temperature sensitivity of cell division. Genetic analysis characterized two new genetic loci, acrC and acrD. The former was mapped between tonA and proA, and the latter between the origin of genetic transfer of HfrH and serB. acrC and acrD mutants could divide but did not initiate a new round of deoxyribonucleic acid (DNA) replication at 43 degrees C. DNA synthesis of the acrC mutant cells ceased after a period of time following temperature shift-up, and thereafter DNA degradation occurred. However, cell mass continued to increase for a long time at the nonpermissive temperature. On the other hand, DNA synthesis of the acrD mutant cells ceased soon after the shift-up, and the cell mass did not appreciably increase during the prolonged incubation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Donch J., Greenberg J. Ultraviolet sensitivity gene of Escherichia coli B. J Bacteriol. 1968 May;95(5):1555–1559. doi: 10.1128/jb.95.5.1555-1559.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fico R. M., Chen T. K., Canellakis E. S. Bifunctional intercalators: relationship of antitumor activity of diacridines to the cell membrane. Science. 1977 Oct 7;198(4312):53–56. doi: 10.1126/science.897680. [DOI] [PubMed] [Google Scholar]
  4. HAYES W. The mechanism of genetic recombination in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1953;18:75–93. doi: 10.1101/sqb.1953.018.01.016. [DOI] [PubMed] [Google Scholar]
  5. Hirota Y., Ryter A., Jacob F. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol. 1968;33:677–693. doi: 10.1101/sqb.1968.033.01.077. [DOI] [PubMed] [Google Scholar]
  6. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  7. LERMAN L. S. The structure of the DNA-acridine complex. Proc Natl Acad Sci U S A. 1963 Jan 15;49:94–102. doi: 10.1073/pnas.49.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nakamura H. Acriflavine-binding capacity of Escherichia coli in relation to acriflavine sensitivity and metabolic activity. J Bacteriol. 1966 Nov;92(5):1447–1452. doi: 10.1128/jb.92.5.1447-1452.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nakamura H. Gene-Controlled Resistance to Acriflavine and Other Basic Dyes in Escherichia coli. J Bacteriol. 1965 Jul;90(1):8–14. doi: 10.1128/jb.90.1.8-14.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakamura H. Genetic determination of resistance to acriflavine, phenethyl alcohol, and sodium dodecyl sulfate in Escherichia coli. J Bacteriol. 1968 Oct;96(4):987–996. doi: 10.1128/jb.96.4.987-996.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nakamura H., Hachiya N., Tojo T. Second acriflavine sensitivity mutation, acrB, in Escherichia coli K-12. J Bacteriol. 1978 Jun;134(3):1184–1187. doi: 10.1128/jb.134.3.1184-1187.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakamura H. Plasmid-instability in acrA mutants of Escherichia coli K12. J Gen Microbiol. 1974 Sep;84(1):85–93. doi: 10.1099/00221287-84-1-85. [DOI] [PubMed] [Google Scholar]
  13. Nakamura H., Suganuma A. Membrane mutation associated with sensitivity to acriflavine in Escherichia coli. J Bacteriol. 1972 Apr;110(1):329–335. doi: 10.1128/jb.110.1.329-335.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nakamura H., Tojo T., Greenberg J. Interaction of the expression of two membrane genes, acrA and plsA, in Escherichia coli K-12. J Bacteriol. 1975 Jun;122(3):874–879. doi: 10.1128/jb.122.3.874-879.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ricard M., Hirota Y. Process of cellular division in Escherichia coli: physiological study on thermosensitive mutants defective in cell division. J Bacteriol. 1973 Oct;116(1):314–322. doi: 10.1128/jb.116.1.314-322.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Silver S., Levine E., Spielman P. M. Acridine binding by Escherichia coli: pH dependency and strain differences. J Bacteriol. 1968 Feb;95(2):333–339. doi: 10.1128/jb.95.2.333-339.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TUBBS R. K., DITMARS W. E., Jr, VANWINKLE Q. HETEROGENEITY OF THE INTERACTION OF DNA WITH ACRIFLAVINE. J Mol Biol. 1964 Aug;9:545–557. doi: 10.1016/s0022-2836(64)80226-6. [DOI] [PubMed] [Google Scholar]
  18. Taylor A L, Adelberg E A. Linkage Analysis with Very High Frequency Males of Escherichia Coli. Genetics. 1960 Sep;45(9):1233–1243. doi: 10.1093/genetics/45.9.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weisblum B., De Haseth P. L. Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylaterich regions in DNA. Proc Natl Acad Sci U S A. 1972 Mar;69(3):629–632. doi: 10.1073/pnas.69.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]

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