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. 1979 Jul;139(1):137–140. doi: 10.1128/jb.139.1.137-140.1979

Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage E79.

A F Morgan
PMCID: PMC216838  PMID: 110777

Abstract

A mutant of the virulent bacteriophage E79 was isolated which mediated generalized transduction in Pseudomonas aeruginosa. Variable recovery of transductants as a result of phage killing was avoided by the use of recipients carrying the IncP-2 plasmid R38, and transduction frequencies of 4 X 10(-6) to 1 X 10(-5) per plaque-forming unit were obtained. Linkage studies have indicated that the coinheritance frequencies are less than would be expected from the published molecular weight of E79 deoxyribonucleic acid (120 X 10(6). By using recipients carrying R38, low-frequency transduction by wild-type E79 and two other virulent phages, F8 and phi 16, was demonstrated.

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Selected References

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  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bryan L. E., Semaka S. D., Van den Elzen H. M., Kinnear J. E., Whitehouse R. L. Characteristics of R931 and other Pseudomonas aeruginosa R factors. Antimicrob Agents Chemother. 1973 May;3(5):625–637. doi: 10.1128/aac.3.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carrigan J. M., Helman Z. M., Krishnapillai V. Transfer-deficient mutants of the narrow-host-range plasmid R91-5 of Pseudomonas aeruginosa. J Bacteriol. 1978 Sep;135(3):911–919. doi: 10.1128/jb.135.3.911-919.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandler P. M., Krishnapillai V. Isolation and properties of recombination-deficient mutants of Pseudomonas aeruginosa. Mutat Res. 1974 Apr;23(1):15–23. doi: 10.1016/0027-5107(74)90155-9. [DOI] [PubMed] [Google Scholar]
  5. DAVISON P. F., FREIFELDER D., HOLLOWAY B. W. INTERRUPTIONS IN THE POLYNUCLEOTIDE STRANDS IN BACTERIOPHAGE DNA. J Mol Biol. 1964 Jan;8:1–10. doi: 10.1016/s0022-2836(64)80142-x. [DOI] [PubMed] [Google Scholar]
  6. Drexler H. Transduction by bacteriophage T1. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1083–1088. doi: 10.1073/pnas.66.4.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ely B., Johnson R. C. Generalized Transduction in CAULOBACTER CRESCENTUS. Genetics. 1977 Nov;87(3):391–399. doi: 10.1093/genetics/87.3.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FARGIE B., HOLLOWAY B. W. ABSENCE OF CLUSTERING OF FUNCTIONALLY RELATED GENES IN PSEUDOMONAS AERUGINOSA. Genet Res. 1965 Jul;6:284–299. doi: 10.1017/s0016672300004158. [DOI] [PubMed] [Google Scholar]
  9. HOLLOWAY B. W., EGAN J. B., MONK M. Lysogeny in Pseudomonas aeruginosa. Aust J Exp Biol Med Sci. 1960 Aug;38:321–329. doi: 10.1038/icb.1960.34. [DOI] [PubMed] [Google Scholar]
  10. Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
  11. Haas D., Holloway B. W., Schamböck A., Leisinger T. The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa. Mol Gen Genet. 1977 Jul 7;154(1):7–22. doi: 10.1007/BF00265571. [DOI] [PubMed] [Google Scholar]
  12. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Isaac J. H., Holloway B. W. Control of arginine biosynthesis in Pseudomonas aeruginosa. J Gen Microbiol. 1972 Dec;73(3):427–438. doi: 10.1099/00221287-73-3-427. [DOI] [PubMed] [Google Scholar]
  14. Isaac J. H., Holloway B. W. Control of pyrimidine biosynthesis in Pseudomonas aeruginosa. J Bacteriol. 1968 Nov;96(5):1732–1741. doi: 10.1128/jb.96.5.1732-1741.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jacoby G. A. Properties of R plasmids determining gentamicin resistance by acetylation in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1974 Sep;6(3):239–252. doi: 10.1128/aac.6.3.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Krishnapillai V. A novel transducing phage. Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa. Mol Gen Genet. 1972;114(2):134–143. doi: 10.1007/BF00332784. [DOI] [PubMed] [Google Scholar]
  17. Krishnapillai V. The use of bacteriophages for differentiating plasmids of Pseudomonas aeruginosa. Genet Res. 1974 Jun;23(3):327–334. doi: 10.1017/s0016672300014968. [DOI] [PubMed] [Google Scholar]
  18. Mee B. J., Lee B. T. An analysis of histidine requiring mutants in Pseudomonas aeruginosa. Genetics. 1967 Apr;55(4):709–722. doi: 10.1093/genetics/55.4.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schmieger H. A method for detection of phage mutants with altered transducing ability. Mol Gen Genet. 1971;110(4):378–381. doi: 10.1007/BF00438281. [DOI] [PubMed] [Google Scholar]
  20. Schmieger H. Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet. 1972;119(1):75–88. doi: 10.1007/BF00270447. [DOI] [PubMed] [Google Scholar]
  21. Stanisich V. A., Holloway B. W. A mutant sex factor of Pseudomonas aeruginosa. Genet Res. 1972 Feb;19(1):91–108. doi: 10.1017/s0016672300014294. [DOI] [PubMed] [Google Scholar]
  22. Voellym R., Leisinger T. Role of 4-aminobutyrate aminotransferase in the arginine metabolism of Pseudomonas aeruginosa. J Bacteriol. 1976 Dec;128(3):722–729. doi: 10.1128/jb.128.3.722-729.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wall J. D., Harriman P. D. Phage P1 mutants with altered transducing abilities for Escherichia coli. Virology. 1974 Jun;59(2):532–544. doi: 10.1016/0042-6822(74)90463-2. [DOI] [PubMed] [Google Scholar]
  24. Watson J. M., Holloway B. W. Suppressor mutations in Pseudomonas aeruginosa. J Bacteriol. 1976 Mar;125(3):780–786. doi: 10.1128/jb.125.3.780-786.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yasbin R. E., Young F. E. Transduction in Bacillus subtilis by bacteriophage SPP1. J Virol. 1974 Dec;14(6):1343–1348. doi: 10.1128/jvi.14.6.1343-1348.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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