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. 1979 Jul;139(1):239–246. doi: 10.1128/jb.139.1.239-246.1979

Gap-filling repair synthesis induced by ultraviolet light in a Bacillus subtilis Uvr- mutant.

C T Hadden
PMCID: PMC216851  PMID: 110780

Abstract

Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthesis. One of the mutations (uvs-42) resulted in a severe inhibition of incision, dimer excision, and repair synthesis. In contrast, the other mutant (uvr-1) slowly incised and excised dimers and did repair synthesis in patches which appear to be several-fold longer than those in the wild-type strain, apparently because large gaps are produced at excision sites. The results indicate that the primary defect in uvs-42 cells is in initiation of dimer excision, whereas the uvr-1 mutation appears to be a defect in the exonuclease normally used to complete dimer excision.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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