Viral Proteomics

Volume 71, no. 2, p. 398-411, 2007. Page 400, column 1, lines 10-11: The statement that “there are no reports of virion proteomic studies of any alphaherpesviruses…” is incorrect, as a proteomic analysis has been conducted on pseudorabies virus particles. K. Michael, B. G. Klupp, T. C. Mettenleiter, and A. Karger (J. Virol. 80:1332-13391, 2006) used a SILAC approach coupled with 1D and 2D gel electrophoresis to compare protein levels in wild-type pseudorabies virions to those generated by mutant viruses lacking specific genes. This led to the identification of 22 viral and 4 cellular proteins (actin, HSP70, annexin A1, and annexin A2), some of which were altered in abundance in the mutant viruses. In particular, the amount of cellular actin in the virion was found to increase upon deletion of each of three major viral tegument proteins.