Abstract
Pleiotropic effects of two ribosomal mutations for cycloheximide resistance were studied in a double-resistant homocaryon. The results obtained indicated that the combination of the two ribosomal mutations results in: (i) morphological abnormalities which suggest a severe distortion in the extension of cell walls and membranes; (ii) disturbance in the normal 60S-40S subunit ratio; (iii) decreased rate of cell mass production not necessarily associated with inbalance in the 60S-40S ratio; and (iv) cold sensitivity which does not interfere with mycelial mass production.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Graham R. S., Bott K. F. Antibiotic-resistant mutants of Bacillus subtilis conditional for sporulation. Mol Gen Genet. 1975;137(3):227–237. doi: 10.1007/BF00333018. [DOI] [PubMed] [Google Scholar]
- HSU K. S. THE GENETIC BASIS OF ACTIDIONE RESISTANCE IN NEUROSPORA. J Gen Microbiol. 1963 Sep;32:341–347. doi: 10.1099/00221287-32-3-341. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Leighton T. Sporulation-specific translational discrimination in Bacillus subtilis. J Mol Biol. 1974 Jul 15;86(4):855–863. doi: 10.1016/0022-2836(74)90358-1. [DOI] [PubMed] [Google Scholar]
- Martin T. E., Rolleston F. S., Low R. B., Wool I. G. Dissociation and reassociation of skeletal muscle ribosomes. J Mol Biol. 1969 Jul 14;43(1):135–149. doi: 10.1016/0022-2836(69)90084-9. [DOI] [PubMed] [Google Scholar]
- Mishra N. C. Genetics and biochemistry of morphogenesis in Neurospora. Adv Genet. 1977;19:341–405. doi: 10.1016/s0065-2660(08)60248-5. [DOI] [PubMed] [Google Scholar]
- Mizuno T., Yamagata H., Mizushima S. Interaction of cytoplasmic membrane and ribosomes in Escherichia coli: spectinomycin-induced disappearance of membrane protein I-19. J Bacteriol. 1977 Jan;129(1):326–332. doi: 10.1128/jb.129.1.326-332.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rao S. S., Grollman A. P. Cycloheximide resistance in yeast: a property of the 60s ribosomal subunit. Biochem Biophys Res Commun. 1967 Dec 15;29(5):696–704. doi: 10.1016/0006-291x(67)90273-2. [DOI] [PubMed] [Google Scholar]
- Tipper D. J., Johnson C. W., Ginther C. L., Leighton T., Wittmann H. G. Erythromycin resistant mutations in Bacillus subtilis cause temperature sensitive sporulation. Mol Gen Genet. 1977 Jan 18;150(2):147–159. doi: 10.1007/BF00695395. [DOI] [PubMed] [Google Scholar]
- Udem S. A., Warner J. R. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. doi: 10.1016/0022-2836(72)90279-3. [DOI] [PubMed] [Google Scholar]
- Vomvoyanni V. Multigenic control of ribosomal properties associated with cycloheximide sensitivity in Neurospora crassa. Nature. 1974 Apr 5;248(448):508–510. doi: 10.1038/248508a0. [DOI] [PubMed] [Google Scholar]