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. 1981 Jun;146(3):833–840. doi: 10.1128/jb.146.3.833-840.1981

Parasexual genetic analysis of Candida albicans by spheroplast fusion.

R Poulter, K Jeffery, M J Hubbard, M G Shepherd, P A Sullivan
PMCID: PMC216933  PMID: 7016832

Abstract

Doubly auxotrophic strains of Candida albicans were selected from mutagenized cultures. Spheroplasts prepared from the auxotrophic strains were fused with polyethylene glycol. Prototrophic derivatives formed by this fusion protocol from auxotrophic strains were selected by complementation on minimal medium. These prototrophs had a cell volume twice that of the original strain and were shown to be heterozygous at four loci. Prototrophs obtained by this procedure infrequently gave rise to auxotrophic recombinants whose cell volume remained twice that of the original strain. It is suggested that these auxotrophic recombinants arise from mitotic crossing-over. This paper is the first report of a parasexual cycle in C. albicans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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