Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Jun;146(3):902–906. doi: 10.1128/jb.146.3.902-906.1981

Genetic and biochemical requirements for chemotaxis to L-proline in Escherichia coli.

M Clancy, K A Madill, J M Wood
PMCID: PMC216942  PMID: 7016836

Abstract

Chemotaxis to L-proline was examined by the capillary assay, using a set of Escherichia coli strains bearing well-defined defects in the enzymes of proline transport and utilization. Aspartate taxis was measured as a constitutive, control activity whose receptor and transducer requirements are known. Proline chemotaxis showed a pattern of induction more analogous to that of proline dehydrogenase than of that of proline transport, but chemotaxis to proline was eliminated by mutations eliminating either or both of these activities. No response to proline was observed in the absence of a proline concentration gradient or when succinate was provided as an oxidizable carbon source. These data suggest that the chemotactic response to proline results from a direct impact of proline oxidation on the energy metabolism of the cell.

Full text

PDF
902

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. doi: 10.1099/00221287-74-1-77. [DOI] [PubMed] [Google Scholar]
  2. Anderson R. R., Menzel R., Wood J. M. Biochemistry and regulation of a second L-proline transport system in Salmonella typhimurium. J Bacteriol. 1980 Mar;141(3):1071–1076. doi: 10.1128/jb.141.3.1071-1076.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berg H. C., Brown D. A. Chemotaxis in Escherichia coli analysed by three-dimensional tracking. Nature. 1972 Oct 27;239(5374):500–504. doi: 10.1038/239500a0. [DOI] [PubMed] [Google Scholar]
  5. Clarke S., Koshland D. E., Jr Membrane receptors for aspartate and serine in bacterial chemotaxis. J Biol Chem. 1979 Oct 10;254(19):9695–9702. [PubMed] [Google Scholar]
  6. Khan S., Macnab R. M. Proton chemical potential, proton electrical potential and bacterial motility. J Mol Biol. 1980 Apr 15;138(3):599–614. doi: 10.1016/s0022-2836(80)80019-2. [DOI] [PubMed] [Google Scholar]
  7. Khan S., Macnab R. M. The steady-state counterclockwise/clockwise ratio of bacterial flagellar motors is regulated by protonmotive force. J Mol Biol. 1980 Apr 15;138(3):563–597. doi: 10.1016/s0022-2836(80)80018-0. [DOI] [PubMed] [Google Scholar]
  8. Laszlo D. J., Taylor B. L. Aerotaxis in Salmonella typhimurium: role of electron transport. J Bacteriol. 1981 Feb;145(2):990–1001. doi: 10.1128/jb.145.2.990-1001.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Macnab R. M. Bacterial motility and chemotaxis: the molecular biology of a behavioral system. CRC Crit Rev Biochem. 1978;5(4):291–341. doi: 10.3109/10409237809177145. [DOI] [PubMed] [Google Scholar]
  10. Mesibov R., Adler J. Chemotaxis toward amino acids in Escherichia coli. J Bacteriol. 1972 Oct;112(1):315–326. doi: 10.1128/jb.112.1.315-326.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mesibov R., Ordal G. W., Adler J. The range of attractant concentrations for bacterial chemotaxis and the threshold and size of response over this range. Weber law and related phenomena. J Gen Physiol. 1973 Aug;62(2):203–223. doi: 10.1085/jgp.62.2.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller J. B., Koshland D. E., Jr Protonmotive force and bacterial sensing. J Bacteriol. 1980 Jan;141(1):26–32. doi: 10.1128/jb.141.1.26-32.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scarpulla R. C., Soffer R. L. Membrane-bound proline dehydrogenase from Escherichia coli. Solubilization, purification, and characterization. J Biol Chem. 1978 Sep 10;253(17):5997–6001. [PubMed] [Google Scholar]
  14. Shioi J. I., Imae Y., Oosawa F. Protonmotive force and motility of Bacillus subtilis. J Bacteriol. 1978 Mar;133(3):1083–1088. doi: 10.1128/jb.133.3.1083-1088.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spudich J. L., Koshland D. E., Jr Quantitation of the sensory response in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1975 Feb;72(2):710–713. doi: 10.1073/pnas.72.2.710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stavrianopoulos J. G., Karkas J. D., Chargaff E. Mechanism of DNA replication by highly purified DNA polymerase of chicken embryo. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2609–2613. doi: 10.1073/pnas.69.9.2609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Szmelcman S., Adler J. Change in membrane potential during bacterial chemotaxis. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4387–4391. doi: 10.1073/pnas.73.12.4387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taylor B. L., Miller J. B., Warrick H. M., Koshland D. E., Jr Electron acceptor taxis and blue light effect on bacterial chemotaxis. J Bacteriol. 1979 Nov;140(2):567–573. doi: 10.1128/jb.140.2.567-573.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wood J. M., Zadworny D. Amplification of the put genes and identification of the put gene products in Escherichia coli K12. Can J Biochem. 1980 Oct;58(10):787–796. doi: 10.1139/o80-110. [DOI] [PubMed] [Google Scholar]
  20. Wood J. M., Zadworny D. Characterization of an inducible porter required for L-proline catabolism by Escherichia coli K12. Can J Biochem. 1979 Oct;57(10):1191–1199. doi: 10.1139/o79-155. [DOI] [PubMed] [Google Scholar]
  21. de Jong M. H., van der Drift C. Control of the chemotactic behavior of Bacillus subtilis cells. Arch Microbiol. 1978 Jan 23;116(1):1–8. doi: 10.1007/BF00408727. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES