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. 1981 Jun;146(3):1098–1105. doi: 10.1128/jb.146.3.1098-1105.1981

The araIc mutation in Escherichia coli B/r.

L G Cass, A H Horwitz, G Wilcox
PMCID: PMC216966  PMID: 6263857

Abstract

The araIc allele is a cis-acting mutation which has been used to define the araBAD promoter in Escherichia coli B/r. Nineteen araIc mutants were originally isolated by Englesberg and co-workers as Ara+ "revertants" of an araC deletion mutant (Englesberg et al. J. Mol. Biol. 43:281-298, 1969). The mutants constitutively expressed araBAD gene products in the absence of functional araC activator protein. Eight of the araIc mutations have been cloned by in vivo recombination onto pBR322-ara hybrid plasmids. Restriction and DNA sequence analysis of these araIc mutations showed that they result from a single base-pair change located at -35 in the araBAD promoter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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