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. 1981 Jun;146(3):1117–1123. doi: 10.1128/jb.146.3.1117-1123.1981

Sodium dodecyl sulfate-sensitive septation in a mitomycin C-sensitive, mtc, mutant of Escherichia coli.

T Higashi, K Suzuki, N Otsuji
PMCID: PMC216968  PMID: 6787013

Abstract

A mitomycin C-sensitive, mtc, mutant of Escherichia coli has an altered cell surface and is sensitive to sodium dodecyl sulfate (SDS). The mutant, M27, formed multinucleate nonseptated filaments in the presence of a low concentration of SDS (50 microgram/ml). When the culture grown at that concentration of SDS was diluted with an SDS-free medium, the filaments began to divide at a very rapid rate after a lag of about 20 min. Chloramphenicol inhibited this recovery division when added within 10 min after SDS dilution but did not inhibit the division when added 20 min after dilution. Penicillin G at a low concentration, which is enough to cause filamentation, had virtually no effect on the recovery division of SDS-induced filaments. The division of penicillin G-induced filaments was inhibited by SDS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADLER H. I., HARDIGREE A. A. ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Mar;87:720–726. doi: 10.1128/jb.87.3.720-726.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burdett I. D., Murray R. G. Electron microscope study of septum formation in Escherichia coli strains B and B-r during synchronous growth. J Bacteriol. 1974 Sep;119(3):1039–1056. doi: 10.1128/jb.119.3.1039-1056.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burdett I. D., Murray R. G. Septum formation in Escherichia coli: characterization of septal structure and the effects of antibiotics on cell division. J Bacteriol. 1974 Jul;119(1):303–324. doi: 10.1128/jb.119.1.303-324.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dame J. B., Shapiro B. M. Use of polymyxin B, levallorphan, and tetracaine to isolate novel envelope mutants of Escherichia coli. J Bacteriol. 1976 Aug;127(2):961–972. doi: 10.1128/jb.127.2.961-972.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donachie W. D., Begg K. J. Growth of the bacterial cell. Nature. 1970 Sep 19;227(5264):1220–1224. doi: 10.1038/2271220a0. [DOI] [PubMed] [Google Scholar]
  6. Donachie W. D., Begg K. J., Lutkenhaus J. F., Salmond G. P., Martinez-Salas E., Vincente M. Role of the ftsA gene product in control of Escherichia coli cell division. J Bacteriol. 1979 Nov;140(2):388–394. doi: 10.1128/jb.140.2.388-394.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Filip C., Fletcher G., Wulff J. L., Earhart C. F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973 Sep;115(3):717–722. doi: 10.1128/jb.115.3.717-722.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fletcher G., Irwin C. A., Henson J. M., Fillingim C., Malone M. M., Walker J. R. Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages. J Bacteriol. 1978 Jan;133(1):91–100. doi: 10.1128/jb.133.1.91-100.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guerola N., Ingraham J. L., Cerdá-Olmedo E. Induction of closely linked multiple mutations by nitrosoguanidine. Nat New Biol. 1971 Mar 24;230(12):122–125. doi: 10.1038/newbio230122a0. [DOI] [PubMed] [Google Scholar]
  10. Hirota Y., Ryter A., Jacob F. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol. 1968;33:677–693. doi: 10.1101/sqb.1968.033.01.077. [DOI] [PubMed] [Google Scholar]
  11. Hoffmann B., Messer W., Schwarz U. Regulation of polar cap formation in the life cycle of Escherichia coli. J Supramol Struct. 1972;1(1):29–37. doi: 10.1002/jss.400010105. [DOI] [PubMed] [Google Scholar]
  12. Inuzuka N., Nakamura S., Inuzuka M., Tomoeda M. Specific action of sodium dodecyl sulfate on the sex factor of Escherichia coli K-12 Hfr strains. J Bacteriol. 1969 Nov;100(2):827–835. doi: 10.1128/jb.100.2.827-835.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jones N. C., Donachie W. D. Chromosome replication, transcription and control of cell division in Escherichia coli. Nat New Biol. 1973 May 23;243(125):100–103. [PubMed] [Google Scholar]
  14. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lazdunski C., Shaprio B. M. Relationship between permeability, cell division, and murein metabolism in a mutant of Escherichia coli. J Bacteriol. 1972 Aug;111(2):499–509. doi: 10.1128/jb.111.2.499-509.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lutkenhaus J. F., Donachie W. D. Identification of the ftsA gene product. J Bacteriol. 1979 Mar;137(3):1088–1094. doi: 10.1128/jb.137.3.1088-1094.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nagai K., Tamura G. Mutant of Escherichia coli with thermosensitive protein in the process of cellular division. J Bacteriol. 1972 Nov;112(2):959–966. doi: 10.1128/jb.112.2.959-966.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nakamura H. Genetic determination of resistance to acriflavine, phenethyl alcohol, and sodium dodecyl sulfate in Escherichia coli. J Bacteriol. 1968 Oct;96(4):987–996. doi: 10.1128/jb.96.4.987-996.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  20. Otsuji N., Higashi T., Kawamata J. Genetic and physiological analysis of mitomycin C-sensitive mutants of Escherichia coli K12. Biken J. 1972 Jun;15(2):49–59. [PubMed] [Google Scholar]
  21. Otsuji N., Iyehara H., Hideshima Y. Isolation and characterization of an Escherichia coli ruv mutant which forms nonseptate filaments after low doses of ultraviolet light irradiation. J Bacteriol. 1974 Feb;117(2):337–344. doi: 10.1128/jb.117.2.337-344.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Otsuji N. Properties of mitomycin C-sensitive mutants of Escherichia coli K-12. J Bacteriol. 1968 Feb;95(2):540–545. doi: 10.1128/jb.95.2.540-545.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. RYTER A., KELLENBERGER E., BIRCHANDERSEN A., MAALOE O. Etude au microscope électronique de plasmas contenant de l'acide désoxyribonucliéique. I. Les nucléoides des bactéries en croissance active. Z Naturforsch B. 1958 Sep;13B(9):597–605. [PubMed] [Google Scholar]
  24. Reeve J. N., Groves D. J., Clark D. J. Regulation of Cell Division in Escherichia coli: Characterization of Temperature-Sensitive Division Mutants. J Bacteriol. 1970 Dec;104(3):1052–1064. doi: 10.1128/jb.104.3.1052-1064.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ricard M., Hirota Y. Process of cellular division in Escherichia coli: physiological study on thermosensitive mutants defective in cell division. J Bacteriol. 1973 Oct;116(1):314–322. doi: 10.1128/jb.116.1.314-322.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Santos D., De Almeida D. F. Isolation and characterization of a new temperature-sensitive cell division mutant of Escherichia coli K-12. J Bacteriol. 1975 Dec;124(3):1502–1507. doi: 10.1128/jb.124.3.1502-1507.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schnaitman C. A. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J Bacteriol. 1970 Nov;104(2):890–901. doi: 10.1128/jb.104.2.890-901.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schnaitman C. A. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. J Bacteriol. 1971 Oct;108(1):545–552. doi: 10.1128/jb.108.1.545-552.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schwarz U., Asmus A., Frank H. Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969 May 14;41(3):419–429. doi: 10.1016/0022-2836(69)90285-x. [DOI] [PubMed] [Google Scholar]
  30. Spratt B. G. Temperature-sensitive cell division mutants of Escherichia coli with thermolabile penicillin-binding proteins. J Bacteriol. 1977 Jul;131(1):293–305. doi: 10.1128/jb.131.1.293-305.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tormo A., Martínez-Salas E., Vicente M. Involvement of the ftsA gene product in late stages of the Escherichia coli cell cycle. J Bacteriol. 1980 Feb;141(2):806–813. doi: 10.1128/jb.141.2.806-813.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. VAN DE PUTTE P., VAN DILLEWIJN, ROERSCH A. THE SELECTION OF MUTANTS OF ESCHERICHIA COLI WITH IMPAIRED CELL DIVISION AT ELEVATED TEMPERATURE. Mutat Res. 1964 Jul;106:121–128. doi: 10.1016/0027-5107(64)90014-4. [DOI] [PubMed] [Google Scholar]
  33. Walker J. R., Kovarik A., Allen J. S., Gustafson R. A. Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation. J Bacteriol. 1975 Aug;123(2):693–703. doi: 10.1128/jb.123.2.693-703.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wijsman H. J., Koopman C. R. The relation of the genes envA and ftsA in Escherichia coli. Mol Gen Genet. 1976 Aug 10;147(1):99–102. doi: 10.1007/BF00337942. [DOI] [PubMed] [Google Scholar]
  35. Woldringh C. L. Lysis of the cell membrane of Escherichia coli K12 by ionic detergents. Biochim Biophys Acta. 1970 Nov 12;224(1):288–290. doi: 10.1016/0005-2787(70)90650-7. [DOI] [PubMed] [Google Scholar]
  36. Woldringh C. L., van Iterson W. Effects of treatment with sodium dodecyl sulfate on the ultrastructure of Escherichia coli. J Bacteriol. 1972 Sep;111(3):801–813. doi: 10.1128/jb.111.3.801-813.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wu P. C., Pardee A. B. Cell division of Escherichia coli: control by membrane organization. J Bacteriol. 1973 May;114(2):603–611. doi: 10.1128/jb.114.2.603-611.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Zusman D. R., Inouye M., Pardee A. B. Cell division in Escherichia coli: evidence for regulation of septation by effector molecules. J Mol Biol. 1972 Aug 14;69(1):119–136. doi: 10.1016/0022-2836(72)90027-7. [DOI] [PubMed] [Google Scholar]

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