Abstract
Substantial amounts of exogenously supplied cholesterol were incorporated into the membranes of Escherichia coli during growth and caused a large decrease in membrane fluidity. Although no compensatory changes in fatty acid composition were observed, the incorporation of cholesterol did not affect the rate of growth of E. coli or interfere with the changes in fatty acid composition which normally occur during growth at different temperatures.
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- ARGAMAN M., RAZIN S. CHOLESTEROL AND CHOLESTEROL ESTERS IN MYCOPLASMA. J Gen Microbiol. 1965 Jan;38:153–160. doi: 10.1099/00221287-38-1-153. [DOI] [PubMed] [Google Scholar]
- Allain C. C., Poon L. S., Chan C. S., Richmond W., Fu P. C. Enzymatic determination of total serum cholesterol. Clin Chem. 1974 Apr;20(4):470–475. [PubMed] [Google Scholar]
- Baldassare J. J., Saito Y., Silbert D. F. Effect of sterol depletion on LM cell sterol mutants. Changes in the lipid composition of the plasma membrane and their effects on 3-O-methlglucose transport. J Biol Chem. 1979 Feb 25;254(4):1108–1113. [PubMed] [Google Scholar]
- Baldassare J. J., Silbert D. F. Membrane phospholipid metabolism in response to sterol depletion. Compensatory compositional changes which maintain 3-O-methylglucose transport. J Biol Chem. 1979 Oct 25;254(20):10078–10083. [PubMed] [Google Scholar]
- Berger B., Carty C. E., Ingram L. O. Alcohol-induced changes in the phospholipid molecular species of Escherichia coli. J Bacteriol. 1980 Jun;142(3):1040–1044. doi: 10.1128/jb.142.3.1040-1044.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dahl J. S., Dahl C. E., Bloch K. Sterols in membranes: growth characteristics and membrane properties of Mycoplasma capricolum cultured on cholesterol and lanosterol. Biochemistry. 1980 Apr 1;19(7):1467–1472. doi: 10.1021/bi00548a032. [DOI] [PubMed] [Google Scholar]
- DiRienzo J. M., Inouye M. Lipid fluidity-dependent biosynthesis and assembly of the outer membrane proteins of E. coli. Cell. 1979 May;17(1):155–161. doi: 10.1016/0092-8674(79)90303-9. [DOI] [PubMed] [Google Scholar]
- Fulco A. J. Metabolic alterations of fatty acids. Annu Rev Biochem. 1974;43(0):215–241. doi: 10.1146/annurev.bi.43.070174.001243. [DOI] [PubMed] [Google Scholar]
- Ingram L. O. Adaptation of membrane lipids to alcohols. J Bacteriol. 1976 Feb;125(2):670–678. doi: 10.1128/jb.125.2.670-678.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ingram L. O., Vreeland N. S. Differential effects of ethanol and hexanol on the Escherichia coli cell envelope. J Bacteriol. 1980 Nov;144(2):481–488. doi: 10.1128/jb.144.2.481-488.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Luria S. E., Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. doi: 10.1093/genetics/28.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Okuyama H., Yamada K., Kameyama Y., Ikezawa H., Akamatsu Y., Nojima S. Regulation of membrane lipid synthesis in Escherichia coli after shifts in temperature. Biochemistry. 1977 Jun 14;16(12):2668–2673. doi: 10.1021/bi00631a013. [DOI] [PubMed] [Google Scholar]
- Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
- Razin S. Cholesterol incorporation into bacterial membranes. J Bacteriol. 1975 Oct;124(1):570–572. doi: 10.1128/jb.124.1.570-572.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Razin S., Tourtellotte M. E., McElhaney R. N., Pollack J. D. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J Bacteriol. 1966 Feb;91(2):609–616. doi: 10.1128/jb.91.2.609-616.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rintoul D. A., Chou S. M., Silbert D. F. Physical characterization of sterol-depleted LM-cell plasma membranes. J Biol Chem. 1979 Oct 25;254(20):10070–10077. [PubMed] [Google Scholar]
- Rottem S., Yashouv J., Ne'eman Z., Razin S. Cholesterol in mycoplasma membranes. Composition, ultrastructure and biological properties of membranes from Mycoplasma mycoides var. capri cells adapted to grow with low cholesterol concentrations. Biochim Biophys Acta. 1973 Nov 16;323(4):495–508. doi: 10.1016/0005-2736(73)90158-2. [DOI] [PubMed] [Google Scholar]
- Saito Y., Silbert D. F. Selective effects of membrane sterol depletion on surface function thymidine and 3-O-methyl-D-glucose transport in a sterol auxotroph. J Biol Chem. 1979 Feb 25;254(4):1102–1107. [PubMed] [Google Scholar]
- Shaw M. K., Ingraham J. L. Fatty Acid Composition of Escherichia coli as a Possible Controlling Factor of the Minimal Growth Temperature. J Bacteriol. 1965 Jul;90(1):141–146. doi: 10.1128/jb.90.1.141-146.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw M. K., Ingraham J. L. Synthesis of macromolecules by Escherichia coli near the minimal temperature for growth. J Bacteriol. 1967 Jul;94(1):157–164. doi: 10.1128/jb.94.1.157-164.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sinensky M. Homeoviscous adaptation--a homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Feb;71(2):522–525. doi: 10.1073/pnas.71.2.522. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wattenberg B. W., Freter C. E., Silbert D. F. Sterol depletion of LM cells using chemical inhibitors and genetic defects. A comparison of secondary phospholipid alterations. J Biol Chem. 1979 Dec 25;254(24):12295–12298. [PubMed] [Google Scholar]
- de Kruyff B., Demel R. A., van Deenen L. L. The effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intact Acholeplasma laidlawii cell membranes and derived liposomes. Biochim Biophys Acta. 1972 Jan 17;255(1):331–347. doi: 10.1016/0005-2736(72)90032-6. [DOI] [PubMed] [Google Scholar]