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. 1981 Jun;146(3):1154–1157. doi: 10.1128/jb.146.3.1154-1157.1981

Transformation of Azotobacter vinelandii with Plasmids RP4 (IncP-1 Group) and RSF1010 (IncQ Group)

Michel David 1,†, Maurice Tronchet 1,†, Jean Dénarié 1,†
PMCID: PMC216972  PMID: 7016829

Abstract

Multicopy plasmid RSF1010 and four of its in vitro-constructed derivatives were mobilized by the self-transmissible RP4 plasmid into Azotobacter vinelandii UW. Modifications of the Escherichia coli transformation procedure of Cohen et al. (Proc. Natl. Acad. Sci. U.S.A. 69:2110–2114, 1972) allowed transformation of A. vinelandii strains UW and ATCC 12837 with purified RP4 or RSF1010 deoxyribonucleic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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