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. 1981 Jun;146(3):1162–1165. doi: 10.1128/jb.146.3.1162-1165.1981

Cloning restriction fragments that promote expression of a gene in Bacillus subtilis.

D M Williams, E J Duvall, P S Lovett
PMCID: PMC216974  PMID: 6787015

Abstract

Plasmid pPL603 (3.1 megadaltons) specifies neomycin resistance in Bacillus subtilis and contains a structural gene for chloramphenicol acetyltransferase. Cells harboring the plasmid cannot grow on solid media containing 10 microgram of chloramphenicol per ml. Cloning EcoRI (or EcoRI)-generated fragments of deoxyribonucleic acid from several sources into the single EcoRI site in plasmid pPL603, with subsequent selection of transformants of media containing 10 micrograms of chloramphenicol per ml, permits the identification of restriction fragments that promote expression of the chloramphenicol acetyltransferase gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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