Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 May;146(2):713–717. doi: 10.1128/jb.146.2.713-717.1981

Identification of wild-type or mutant alleles of bacterial genes cloned on a bacteriophage lambda vector: isolation of uvrC(am) and other mutants.

J I Auerbach, P Howard-Flanders
PMCID: PMC217016  PMID: 6452445

Abstract

We have identified lambda transducing bacteriophages carrying deoxyribonucleic acid repair or recombination genes of Escherichia coli K-12 by their ability to infect and express their bacterial genes in mutant cells in an agar overlay. This technique has been used to recognize transducing phages carrying uvrC+, ssb+, and other genes and to isolate phages carrying mutant alleles unable to complement ssb or uvrC cells. Several uvrC mutations were obtained which were suppressor sensitive.

Full text

PDF
713

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auerbach J., Howard-Flanders P. The isolation and genetic characteristics of lambda transducing phages of the uvrA+ and uvrC+ genes of E. coli K12. Mol Gen Genet. 1979 Jan 11;168(3):341–344. doi: 10.1007/BF00271506. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blattner F. R., Williams B. G., Blechl A. E., Denniston-Thompson K., Faber H. E., Furlong L., Grunwald D. J., Kiefer D. O., Moore D. D., Schumm J. W. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science. 1977 Apr 8;196(4286):161–169. doi: 10.1126/science.847462. [DOI] [PubMed] [Google Scholar]
  4. Cameron J. R., Panasenko S. M., Lehman I. R., Davis R. W. In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3416–3420. doi: 10.1073/pnas.72.9.3416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chase J. W., Whittier R. F., Auerbach J., Sancar A., Rupp W. D. Amplification of single-strand DNA binding protein in Escherichia coli. Nucleic Acids Res. 1980 Jul 25;8(14):3215–3227. doi: 10.1093/nar/8.14.3215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glassberg J., Meyer R. R., Kornberg A. Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J Bacteriol. 1979 Oct;140(1):14–19. doi: 10.1128/jb.140.1.14-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hong J. S., Ames B. N. Localized mutagenesis of any specific small region of the bacterial chromosome. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158–3162. doi: 10.1073/pnas.68.12.3158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Howard-Flanders P., Boyce R. P., Theriot L. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics. 1966 Jun;53(6):1119–1136. doi: 10.1093/genetics/53.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson B. F. Genetic mapping of the lexC-113 mutation. Mol Gen Genet. 1977 Nov 29;157(1):91–97. doi: 10.1007/BF00268691. [DOI] [PubMed] [Google Scholar]
  10. Kourilsky P., Perricaudet M., Gros D., Garapin A., Gottesman M., Fritsch A., Tiollais P. Description and properties of bacteriophage lambda vectors useful for the cloning of EcoRI DNA fragments. Biochimie. 1978;60(2):183–187. doi: 10.1016/s0300-9084(78)80752-4. [DOI] [PubMed] [Google Scholar]
  11. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marchal C., Greenblatt J., Hofnung M. malB region in Escherichia coli K-12: specialized transducing bacteriophages and first restriction map. J Bacteriol. 1978 Dec;136(3):1109–1119. doi: 10.1128/jb.136.3.1109-1119.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meyer R. R., Glassberg J., Kornberg A. An Escherichia coli mutant defective in single-strand binding protein is defective in DNA replication. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1702–1705. doi: 10.1073/pnas.76.4.1702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shortle D., Koshland D., Weinstock G. M., Botstein D. Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5375–5379. doi: 10.1073/pnas.77.9.5375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vales L. D., Chase J. W., Murphy J. B. Effect of ssbA1 and lexC113 mutations on lambda prophage induction, bacteriophage growth, and cell survival. J Bacteriol. 1980 Aug;143(2):887–896. doi: 10.1128/jb.143.2.887-896.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yamamoto K., Kondo S., Sugimura T. Mechanism of potent mutagenic action of N-methyl-N'-nitro-N-nitrosoguanidine on intracellular phage lambda. J Mol Biol. 1978 Jan 25;118(3):413–430. doi: 10.1016/0022-2836(78)90236-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES