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. 1981 May;146(2):746–754. doi: 10.1128/jb.146.2.746-754.1981

Isolation of cell size mutants of a fission yeast by a new selective method: characterization of mutants and implications for division control mechanisms.

P A Fantes
PMCID: PMC217021  PMID: 7217015

Abstract

Previously known cell size (wee) mutations of fission yeast suppress the mitotic block caused by a defective cdc25 allele. Some 700 revertants of cdc25-22 were obtained after ultraviolet mutagenesis and selection at the restrictive temperature. Most revertants carried the original cdc25 lesion plus a mutation in or very close to the wee1 gene. Two partial wee1 mutations of a new type were found among the revertants. Two new wee mutations mapping at the cdc2 gene (cdc2-w mutants) were also obtained. The various mutations were examined for their effects on cell division size, their efficiency as cdc25 suppressors, and their dominance relations. Full wee1 mutations were found to suppress cdc25 lesions very efficiently, whereas partial wee1 mutations were poor suppressors. The cdc25 suppression ability of cdc2-w mutations was allele specific for cdc2, suggesting bifunctionality of the gene product. The wee1 mutations were recessive for cdc25 suppression; cdc2-w mutations were dominant. A model is proposed for the genetic control of mitotic timing and cell division size, in which the cdc2+ product is needed and is rate limiting for mitosis. The cdc2+ activity is inhibited by the wee1+ product, whereas the cdc25+ product relieves this inhibition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fantes P. A. Control of cell size and cycle time in Schizosaccharomyces pombe. J Cell Sci. 1977 Apr;24:51–67. doi: 10.1242/jcs.24.1.51. [DOI] [PubMed] [Google Scholar]
  2. Fantes P. A., Grant W. D., Pritchard R. H., Sudbery P. E., Wheals A. E. The regulation of cell size and the control of mitosis. J Theor Biol. 1975 Mar;50(1):213–244. doi: 10.1016/0022-5193(75)90034-x. [DOI] [PubMed] [Google Scholar]
  3. Fantes P. Epistatic gene interactions in the control of division in fission yeast. Nature. 1979 May 31;279(5712):428–430. doi: 10.1038/279428a0. [DOI] [PubMed] [Google Scholar]
  4. Gutz H. Site Specific Induction of Gene Conversion in SCHIZOSACCHAROMYCES POMBE. Genetics. 1971 Nov;69(3):317–337. doi: 10.1093/genetics/69.3.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnston G. C., Pringle J. R., Hartwell L. H. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 1;105(1):79–98. doi: 10.1016/0014-4827(77)90154-9. [DOI] [PubMed] [Google Scholar]
  6. Killander D., Zetterberg A. A quantitative cytochemical investigation of the relationship between cell mass and initiation of DNA synthesis in mouse fibroblasts in vitro. Exp Cell Res. 1965 Oct;40(1):12–20. doi: 10.1016/0014-4827(65)90285-5. [DOI] [PubMed] [Google Scholar]
  7. Nurse P. Cell-cycle control in yeasts [proceedings]. Biochem Soc Trans. 1977;5(4):1191–1193. doi: 10.1042/bst0051191. [DOI] [PubMed] [Google Scholar]
  8. Nurse P. Genetic control of cell size at cell division in yeast. Nature. 1975 Aug 14;256(5518):547–551. doi: 10.1038/256547a0. [DOI] [PubMed] [Google Scholar]
  9. Nurse P., Thuriaux P. Controls over the timing of DNA replication during the cell cycle of fission yeast. Exp Cell Res. 1977 Jul;107(2):365–375. doi: 10.1016/0014-4827(77)90358-5. [DOI] [PubMed] [Google Scholar]
  10. Nurse P., Thuriaux P., Nasmyth K. Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1976 Jul 23;146(2):167–178. doi: 10.1007/BF00268085. [DOI] [PubMed] [Google Scholar]
  11. PRESCOTT D. M. Relation between cell growth and cell division. III. Changes in nuclear volume and growth rate and prevention of cell division in Amoeba proteus resulting from cytoplasmic amputations. Exp Cell Res. 1956 Aug;11(1):94–98. doi: 10.1016/0014-4827(56)90193-8. [DOI] [PubMed] [Google Scholar]
  12. Schüpbach M. The isolation and genetic classification of UV-sensitive mutants of Schizosaccharomyces pombe. Mutat Res. 1971 Apr;11(4):361–371. [PubMed] [Google Scholar]
  13. Shields R., Brooks R. F., Riddle P. N., Capellaro D. F., Delia D. Cell size, cell cycle and transition probability in mouse fibroblasts. Cell. 1978 Oct;15(2):469–474. doi: 10.1016/0092-8674(78)90016-8. [DOI] [PubMed] [Google Scholar]
  14. Sudbery P. E., Grant W. D. The control of mitosis in Physarum polycephalum. The effect of lowering the DNA: mass ratio by UV irradiation. Exp Cell Res. 1975 Oct 15;95(2):405–415. doi: 10.1016/0014-4827(75)90566-2. [DOI] [PubMed] [Google Scholar]
  15. Thuriaux P., Nurse P., Carter B. Mutants altered in the control co-ordinating cell division with cell growth in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1978 May 3;161(2):215–220. doi: 10.1007/BF00274190. [DOI] [PubMed] [Google Scholar]

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