Abstract
The addition of coliphage Q beta ribonucleic acid to a cell-free translation system obtained from Coxiella burnetii cells caused the formation of monosomes and disomes from ribosomal subunits and resulted in synthesis of viral coat protein. Rickettsial ribosomes and associated translation factors functioned together, with fidelity, efficiency, and a specificity similar to those of free-living gram-negative bacteria.
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- BOVARNICK M. R., SCHNEIDER L. The incorporation of glycine-1-C14 by typhus rickettsiae. J Biol Chem. 1960 Jun;235:1727–1731. [PubMed] [Google Scholar]
- Baca O. G. Comparison of ribosomes from Coxiella burnetii and Escherichia coli by gel electrophoresis, protein synthesis, and immunological techniques. J Bacteriol. 1978 Oct;136(1):429–432. doi: 10.1128/jb.136.1.429-432.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Capecchi M. R. Cell-free protein synthesis programmed with R17 RNA: identification of two phage proteins. J Mol Biol. 1966 Oct 28;21(1):173–193. doi: 10.1016/0022-2836(66)90086-6. [DOI] [PubMed] [Google Scholar]
- Donahue J. P., Thompson H. A. Protein synthesis in cell-free extracts of Coxiella burnetti. J Gen Microbiol. 1980 Dec;121(2):293–302. doi: 10.1099/00221287-121-2-293. [DOI] [PubMed] [Google Scholar]
- Kronvall G. A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. J Med Microbiol. 1973 May;6(2):187–190. doi: 10.1099/00222615-6-2-187. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Mallavia L. P., Paretsky D. Physiology of rickettsiae. VII. Amino acid incorporation by Coxiella burnetii and by infected hosts. J Bacteriol. 1967 May;93(5):1479–1483. doi: 10.1128/jb.93.5.1479-1483.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson H. A., Baca O. G., Paretsky D. Presence of ribosomal ribonucleic acid in the rickettsia Coxiella burneti. Biochem J. 1971 Nov;125(1):365–366. doi: 10.1042/bj1250365. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss E. Growth and physiology of rickettsiae. Bacteriol Rev. 1973 Sep;37(3):259–283. doi: 10.1128/br.37.3.259-283.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]